ISO 67752 Multi-Class Antimicrobial Residue Analysis in Snacks
The ISO 67752 standard provides a robust and reliable method for the analysis of multi-class antimicrobial residues in snacks. This service is particularly critical for food manufacturers, quality managers, compliance officers, and R&D engineers who need to ensure that their products meet stringent regulatory requirements regarding veterinary drug residues.
Antimicrobials are widely used in livestock production to prevent or control bacterial infections, enhance growth, and improve feed efficiency. However, the presence of these substances in food can pose significant health risks if not properly managed. The ISO 67752 method ensures that all antimicrobial residues are detected at levels below the maximum residue limits (MRLs) specified by regulatory bodies like the European Commission and the World Health Organization.
The analysis process involves several critical steps, starting with the collection of representative samples from various production batches. These samples are then subjected to a series of extraction procedures designed to release all bound and free forms of antimicrobial residues into an aqueous phase. The choice of extraction solvent is crucial, as it must effectively dissolve the residues without causing interference in subsequent analytical steps.
After extraction, the sample undergoes cleanup using solid-phase extraction (SPE) cartridges. This step ensures that only the target analytes are concentrated for analysis while minimizing matrix effects and potential interferences from other compounds present in the snack matrix.
The cleaned extracts are then analyzed using high-performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS). The HPLC system separates the complex mixture of analytes based on their physicochemical properties, while the MS/MS provides definitive identification and quantification of each antimicrobial residue. The use of isotope dilution techniques ensures accurate quantitation even at very low concentrations.
The results are reported in accordance with ISO 67752 guidelines, providing a comprehensive overview of all detected residues along with their respective concentrations relative to the MRLs. This information is invaluable for manufacturers in making informed decisions about process improvements and ensuring product safety.
Our laboratory adheres strictly to international standards such as ISO 67752 to ensure that our results are both accurate and comparable across different laboratories worldwide. By leveraging advanced analytical techniques and experienced personnel, we deliver reliable data that can be trusted by your organization.
- Representative Sample Collection: Ensures the diversity of production batches is captured for analysis.
- Efficient Extraction Methods: Utilizes appropriate solvents to dissolve antimicrobial residues effectively.
- Cleanup with SPE Cartridges: Reduces matrix effects and enhances analytical performance.
- HPLC-MS/MS Analysis: Provides precise separation, identification, and quantification of all detected residues.
In summary, the ISO 67752 method offers a comprehensive approach to detecting multi-class antimicrobial residues in snacks. By adhering strictly to international standards and employing advanced analytical techniques, we provide reliable data that can help your organization ensure product safety and compliance with regulatory requirements.
Applied Standards
The ISO 67752 standard is widely recognized for its stringent criteria in detecting multi-class antimicrobial residues. This standard is specifically designed to cover a broad spectrum of veterinary drugs commonly used in livestock production, including antibiotics, antifungals, and ionophores. The primary purpose of this standard is to ensure that all antimicrobial residues are detected at levels below the MRLs specified by regulatory bodies.
ISO 67752 provides detailed instructions on sample preparation, extraction methods, cleanup procedures, and analytical techniques for HPLC-MS/MS. These guidelines are essential in ensuring consistent and reproducible results across different laboratories. The standard also includes specific criteria for the validation of each method, including linearity, accuracy, precision, and robustness.
By adhering to ISO 67752, our laboratory ensures that all antimicrobial residues are detected at levels below the MRLs specified by regulatory bodies such as the European Commission and the World Health Organization. This standard is particularly important for manufacturers of snacks who need to ensure product safety and compliance with international regulations.
The ISO 67752 method has been validated against numerous veterinary drugs, including:
- Antibiotics: Penicillin, tetracyclines, β-lactams, aminoglycosides, macrolides, quinolones, and sulfonamides.
- Antifungals: Ketoconazole, itraconazole, and fluconazole.
- Ionophores: Monensin, salinomycin, and lasalocid.
Our laboratory strictly adheres to these guidelines to ensure that all antimicrobial residues are detected at levels below the MRLs specified by regulatory bodies. By doing so, we provide reliable data that can be trusted by your organization.
Quality and Reliability Assurance
The ISO 67752 Multi-Class Antimicrobial Residue Analysis in Snacks service is underpinned by rigorous quality assurance measures. Our laboratory is committed to delivering accurate, reliable, and consistent results that meet the highest international standards.
We have implemented a comprehensive Quality Management System (QMS) based on ISO/IEC 17025:2017, which ensures that all aspects of our testing process are systematically controlled and documented. This includes stringent calibration protocols for all analytical instruments, regular maintenance schedules, and ongoing staff training.
Our quality assurance measures extend to the entire testing lifecycle:
- Sample Handling: Samples are stored under optimal conditions to prevent degradation or contamination before analysis.
- Extraction Efficiency: Extraction methods are validated for their ability to recover all target analytes according to ISO 67752 guidelines.
- Cleanup Effectiveness: SPE cartridges are validated for their capacity to remove interfering matrix components while retaining the target analytes.
- Method Validation: Each analytical method is validated using a wide range of spiked samples and quality control samples to ensure accuracy, precision, and robustness.
- Data Integrity: All data are recorded accurately and stored securely in compliance with Good Laboratory Practices (GLP).
We also participate in proficiency testing programs organized by recognized bodies such as the European Co-operation for Accreditation (EA) to validate our analytical capabilities. These programs provide an external assessment of our performance against international standards.
The ISO 67752 method is known for its high reliability, and we take pride in maintaining this reputation through our robust quality assurance protocols. By adhering strictly to these guidelines, we ensure that all antimicrobial residues are detected at levels below the MRLs specified by regulatory bodies. This commitment to quality and reliability is reflected in our consistent performance across various food testing services.
Environmental and Sustainability Contributions
The ISO 67752 Multi-Class Antimicrobial Residue Analysis in Snacks service plays a crucial role in promoting environmental sustainability. By ensuring that all antimicrobial residues are detected at levels below the MRLs specified by regulatory bodies, we contribute to reducing the risk of antimicrobial resistance (AMR) and its associated health risks.
Antimicrobial resistance occurs when bacteria evolve mechanisms to resist the effects of antibiotics, making it more difficult to treat infections. The presence of antimicrobial residues in food can accelerate this process, leading to increased healthcare costs and reduced efficacy of antimicrobial treatments. By detecting these residues at levels below the MRLs, we help mitigate this risk.
The ISO 67752 method also supports sustainability by ensuring that veterinary drugs are used responsibly and efficiently. This reduces unnecessary overuse or misuse of antibiotics, which can lead to environmental contamination and harm to non-target organisms. By adhering to international standards such as ISO/IEC 17025:2017 for our quality management system, we ensure that all aspects of the testing process are systematically controlled and documented.
Our commitment to sustainability extends beyond antimicrobial residues detection. We also contribute to environmental protection by minimizing waste generation during sample preparation and analysis. This is achieved through efficient use of reagents and solvents, recycling of spent materials where possible, and proper disposal of hazardous substances in compliance with local regulations.
In conclusion, the ISO 67752 Multi-Class Antimicrobial Residue Analysis in Snacks service not only ensures product safety but also promotes environmental sustainability. By adhering to international standards and implementing robust quality assurance measures, we contribute to reducing antimicrobial resistance and supporting responsible use of veterinary drugs.
