ISO 13969 Antibiotic Residue Analysis in Milk by HPLC
The analysis of antibiotic residues in milk is a critical procedure to ensure public health and animal welfare. ISO 13969, developed specifically for this purpose, provides robust guidelines for the analysis of antibiotics via High-Performance Liquid Chromatography (HPLC). This service ensures compliance with international standards and regulatory requirements.
Antibiotics used in veterinary medicine can enter milk through various routes, including direct administration to treat infections. Residues above permissible limits can pose serious health risks, especially for infants consuming formula milk. The accurate identification of antibiotic residues is therefore essential for food safety and quality assurance.
The ISO 13969 method employs HPLC with UV detection to separate the antibiotics in a milk sample. After extraction and purification, the samples are injected into the chromatograph, which separates the components based on their retention times. The presence of specific peaks indicates the presence of residues. This process is highly sensitive and can detect even trace amounts.
The service begins with receiving milk samples from clients, followed by a rigorous sample preparation protocol. After extraction and purification steps, the samples are analyzed using an HPLC system equipped with a UV detector. The chromatogram obtained from this analysis provides detailed information about the antibiotic residues present in the milk. This data is then interpreted to determine compliance with regulatory limits.
The HPLC method used for this service adheres strictly to ISO 13969, ensuring accurate and reliable results. It involves several critical steps:
- Sample collection from verified sources
- Precise sample preparation including extraction and purification
- Injection into the HPLC system with subsequent separation of antibiotics
- Data analysis to identify specific peaks indicative of antibiotic residues
The ISO 13969 standard specifies limits for various antibiotics, which are continuously updated based on new research and health concerns. This ensures that the method remains current and relevant.
Our laboratory uses state-of-the-art HPLC equipment to ensure precision and repeatability in every analysis. The system is regularly calibrated and maintained to meet strict quality control standards. Our team of expert scientists and technicians ensures that each sample undergoes meticulous examination, providing clients with accurate results they can trust.
The service also involves a comprehensive reporting process. After completing the HPLC analysis, detailed reports are generated for each client. These reports include:
- Sample identification
- Detailed chromatogram
- List of detected antibiotics and their concentrations
- Evaluation against regulatory limits
- Compliance status with ISO 13969 standards
The results are delivered within a specified timeframe, ensuring that clients receive timely information to make informed decisions regarding their production processes.
Applied Standards
| Standard Number | Description |
|---|---|
| ISO 13969:2017 | Guidelines for the analysis of antibiotics in milk by HPLC |
| ASTM E1851-14 | Standard practice for the determination of antibiotic residues in animal products using high-performance liquid chromatography (HPLC) |
| EN 1276:2013 | Guidelines for the control and reduction of Campylobacter spp. on poultry meat |
The ISO 13969 standard is particularly important as it provides a comprehensive framework for the analysis of antibiotics in milk using HPLC technology. This ensures that all parameters are addressed, from sample preparation to final interpretation of results.
ASTM E1851 and EN 1276 further support our testing by providing additional guidelines on the control and reduction of bacteria in animal products, which can indirectly affect antibiotic residue levels. These standards ensure a holistic approach to food safety and quality assurance.
Industry Applications
- Regulatory compliance for dairy producers and processors
- Evaluation of milk samples from different regions and farms
- Detection of antibiotic residues in raw milk before processing
- Quality control during the production process to ensure product safety
- Verification of compliance with international standards
- Support for R&D to understand residue patterns and mitigation strategies
- Assistance in meeting procurement requirements from food suppliers
The application of this service extends beyond mere compliance. It provides valuable insights into the efficacy of current practices, helping stakeholders make informed decisions about their operations.
Dairy producers can use these results to optimize their production processes and ensure that their products meet stringent quality standards. Processors benefit from knowing the exact levels of antibiotics in raw milk, allowing for adjustments during processing stages.
Customer Impact and Satisfaction
The implementation of ISO 13969 Antibiotic Residue Analysis in Milk by HPLC has a significant impact on our customers. By providing reliable data, we help them comply with international standards and regulations. This ensures that the products they produce are safe for consumption.
Our clients benefit from:
- Avoidance of legal penalties associated with non-compliance
- Better reputation management due to consistent product quality
- Informed decision-making regarding production processes
- Improved customer trust through transparent reporting practices
We strive for high customer satisfaction by delivering accurate, timely results that meet their specific needs. Our team of experts is dedicated to providing personalized support throughout the testing process.
