ISO 35170 Mycotoxin Multi-Class Analysis in Dairy Products
The ISO 35170 standard provides a robust framework for the multi-class analysis of mycotoxins in dairy products, ensuring that food safety and quality standards are met. This service is critical for the dairy sector as it helps maintain product integrity and consumer health. The presence of mycotoxins in dairy products can pose serious risks to human health. By adhering to this standard, laboratories can ensure accurate detection and quantification of multiple mycotoxin classes simultaneously.
The ISO 35170 method employs advanced analytical techniques that are designed to identify a wide range of mycotoxins present in dairy products. It is particularly useful for detecting the following mycotoxin classes: aflatoxins, fumonisins, trichothecenes, zearalenone, and ergot alkaloids. This comprehensive approach allows laboratories to provide clients with precise data on multiple contaminants simultaneously, ensuring a more thorough assessment of product safety.
The service involves several key steps, starting from the collection of samples, which should be representative of the batch or lot being tested. Samples are then prepared according to standard procedures, often involving extraction and cleanup using validated techniques such as QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe). Once prepared, the samples undergo a series of analytical steps that include chromatographic separation followed by mass spectrometric detection.
The use of this standard ensures high levels of precision and accuracy in mycotoxin quantification. The method is designed to detect even trace amounts of mycotoxins, which can be crucial for ensuring compliance with international safety limits set forth by the Codex Alimentarius Commission (CAC) and other regulatory bodies.
| Target Mycotoxins | Quantification Limits |
|---|---|
| Aflatoxins B1, G1, M1 | <0.5 μg/kg |
| Fumonisins B1, B2 | <3 μg/kg |
| Trichothecenes T-2 toxin | <5 μg/kg |
| Zearalenone | <10 μg/kg |
| Ergot alkaloids | <20 μg/kg |
The ISO 35170 method is not only rigorous but also flexible, allowing for adjustments based on the specific needs of different dairy products. This flexibility ensures that even complex matrices can be analyzed accurately and reliably.
Our laboratory uses state-of-the-art instrumentation to perform these analyses, including high-performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS). The combination of HPLC for separation and MS/MS for detection ensures that even very similar compounds can be distinguished accurately. This technology allows us to achieve the required sensitivity and specificity for mycotoxin quantification.
Scope and Methodology
| Target Mycotoxins | Methodology |
|---|---|
| Aflatoxins B1, G1, M1 | HPLC-MS/MS with QuEChERS extraction |
| Fumonisins B1, B2 | HPLC-MS/MS with QuEChERS extraction |
| Trichothecenes T-2 toxin | HPLC-MS/MS with QuEChERS extraction |
| Zearalenone | HPLC-MS/MS with QuEChERS extraction |
| Ergot alkaloids | HPLC-MS/MS with QuEChERS extraction |
The ISO 35170 method is designed to be comprehensive, covering multiple mycotoxin classes in a single analysis. This ensures that all relevant contaminants are detected and quantified simultaneously, providing a holistic view of the mycotoxin profile in dairy products.
The methodology involves several key steps: sample collection, preparation using QuEChERS extraction, chromatographic separation by HPLC, and mass spectrometric detection. Each step is crucial for ensuring accurate results, with careful attention paid to sample handling and instrument calibration.
Industry Applications
- Dairy farms
- Dairy processors
- Retail food safety departments
- Regulatory bodies
This service is particularly valuable for dairy farms and processors who need to ensure the quality of their products. By adhering to ISO 35170, they can demonstrate compliance with international standards and maintain consumer trust. Retail food safety departments also benefit from this service as it helps them enforce strict quality control measures.
Regulatory bodies rely on accurate mycotoxin testing to ensure public health and safety. The ISO 35170 method provides the necessary precision and reliability for regulatory compliance, ensuring that dairy products meet stringent safety standards.
Quality and Reliability Assurance
Our laboratory adheres to stringent quality control measures to ensure the accuracy and reliability of all test results. We have implemented robust quality assurance protocols that include regular calibration of instruments, validation of methods, and participation in proficiency testing programs recognized by industry standards.
