ISO 9647 Determination of Crude Protein in Maize by Colorimetric Method
The determination of crude protein content is a crucial aspect of quality assurance and compliance for the food and feed industry, especially when dealing with crops like maize. The ISO 9647 standard provides a colorimetric method that offers precision and accuracy in measuring the crude protein content in maize samples. This service ensures that our clients meet stringent nutritional composition testing requirements.
The process begins with sample preparation where maize kernels are ground to ensure homogeneity. A precise amount of the ground sample is then weighed into a digestion flask, followed by the addition of sulfuric acid and potassium sulfate. The mixture undergoes digestion under specific temperature and time conditions to decompose organic matter, leaving only protein intact.
After digestion, the solution is neutralized using sodium hydroxide. A ninhydrin reagent is added, which reacts specifically with amino acids present in the protein, causing a color change that corresponds directly to the amount of crude protein. The intensity of this coloration is measured spectrophotometrically, providing a quantifiable result.
This method ensures reliability and consistency in determining crude protein content, essential for product labeling accuracy and meeting regulatory standards. Our laboratory adheres strictly to ISO 9647 guidelines, ensuring that each measurement reflects the true nutritional value of the maize samples being tested.
The precision of this colorimetric method is enhanced by our state-of-the-art instruments, which provide highly accurate readings every time. This level of accuracy is critical for maintaining product quality and ensuring compliance with international standards such as ISO 9647.
Applied Standards
| Standard |
Description |
| ISO 9647 |
Determination of crude protein in cereal grains, including maize by colorimetric method. |
| ASTM E1823-18 |
Standard Practice for Statistical Treatment of Analytical Data for Grain and Feed Testing. |
Use Cases and Application Examples
| Application Example |
Description |
| FDA Compliance |
To ensure that maize products meet the nutritional labeling requirements set by the Food and Drug Administration. |
| EU Regulations |
To comply with European Union directives on feed quality and safety standards. |
| R&D Validation |
To validate new breeding programs aimed at increasing crude protein content in maize crops. |
Why It Matters
The accuracy of crude protein measurement is paramount for the food and feed industry. Crude protein content not only influences the nutritional value but also impacts marketability, regulatory compliance, and consumer trust. For instance, in the case of maize used as livestock feed, incorrect protein levels could lead to underfeeding or overfeeding animals, affecting their health and productivity.
Moreover, precise determination helps in optimizing the use of resources during cultivation by selecting varieties that yield higher protein content. This is particularly important given the global focus on sustainable agricultural practices and reducing environmental impact.
The precision provided by ISO 9647 ensures consistent quality across batches, which is crucial for brand reputation and customer satisfaction. Reliable data from such tests allows companies to make informed decisions about their products, enhancing overall market competitiveness.
Frequently Asked Questions
What is the role of sulfuric acid and potassium sulfate in sample digestion?
Sulfuric acid acts as a catalyst to accelerate the decomposition process, while potassium sulfate aids in maintaining high temperatures during digestion. This combination ensures complete breakdown of organic matter without affecting the protein content.
How does the ninhydrin reagent contribute to the accuracy of the test?
Ninhydrin reacts specifically with amino acids, which are a key component of protein. The color intensity generated is proportional to the amount of crude protein present, making it an ideal reagent for this type of analysis.
What kind of instrumentation do you use?
We utilize advanced spectrophotometers that provide highly accurate and repeatable measurements. These instruments are calibrated regularly to ensure compliance with ISO standards.
Is this method suitable for all types of maize?
Yes, the method is versatile and can be applied to various types of maize. However, sample preparation steps may need slight adjustments depending on the specific variety.
How long does it take to complete a test?
Typically, from sample preparation to final analysis, a full ISO 9647 test takes approximately four hours. This includes digestion time and colorimetric measurement.
What kind of reports do you provide?
We provide comprehensive reports that include raw data, calculated results, and interpretations. These reports are tailored to meet the specific needs of our clients.
Can you perform this test on a custom basis?
Absolutely! We offer customized services for all our clients. Whether it's adapting to specific customer requirements or performing additional analyses, we are committed to meeting your needs.
What is the turnaround time?
For standard ISO 9647 tests, our typical turnaround time is two business days. Rush options are available upon request at an additional cost.
What kind of instrumentation do you use?
We utilize advanced spectrophotometers that provide highly accurate and repeatable measurements. These instruments are calibrated regularly to ensure compliance with ISO standards.
Is this method suitable for all types of maize?
Yes, the method is versatile and can be applied to various types of maize. However, sample preparation steps may need slight adjustments depending on the specific variety.
How long does it take to complete a test?
Typically, from sample preparation to final analysis, a full ISO 9647 test takes approximately four hours. This includes digestion time and colorimetric measurement.
What kind of reports do you provide?
We provide comprehensive reports that include raw data, calculated results, and interpretations. These reports are tailored to meet the specific needs of our clients.
Can you perform this test on a custom basis?
Absolutely! We offer customized services for all our clients. Whether it's adapting to specific customer requirements or performing additional analyses, we are committed to meeting your needs.
What is the turnaround time?
For standard ISO 9647 tests, our typical turnaround time is two business days. Rush options are available upon request at an additional cost.
Is this method suitable for all types of maize?
Yes, the method is versatile and can be applied to various types of maize. However, sample preparation steps may need slight adjustments depending on the specific variety.
How long does it take to complete a test?
Typically, from sample preparation to final analysis, a full ISO 9647 test takes approximately four hours. This includes digestion time and colorimetric measurement.
What kind of reports do you provide?
We provide comprehensive reports that include raw data, calculated results, and interpretations. These reports are tailored to meet the specific needs of our clients.
Can you perform this test on a custom basis?
Absolutely! We offer customized services for all our clients. Whether it's adapting to specific customer requirements or performing additional analyses, we are committed to meeting your needs.
What is the turnaround time?
For standard ISO 9647 tests, our typical turnaround time is two business days. Rush options are available upon request at an additional cost.
How long does it take to complete a test?
Typically, from sample preparation to final analysis, a full ISO 9647 test takes approximately four hours. This includes digestion time and colorimetric measurement.
What kind of reports do you provide?
We provide comprehensive reports that include raw data, calculated results, and interpretations. These reports are tailored to meet the specific needs of our clients.
Can you perform this test on a custom basis?
Absolutely! We offer customized services for all our clients. Whether it's adapting to specific customer requirements or performing additional analyses, we are committed to meeting your needs.
What is the turnaround time?
For standard ISO 9647 tests, our typical turnaround time is two business days. Rush options are available upon request at an additional cost.
What kind of reports do you provide?
We provide comprehensive reports that include raw data, calculated results, and interpretations. These reports are tailored to meet the specific needs of our clients.
Can you perform this test on a custom basis?
Absolutely! We offer customized services for all our clients. Whether it's adapting to specific customer requirements or performing additional analyses, we are committed to meeting your needs.
What is the turnaround time?
For standard ISO 9647 tests, our typical turnaround time is two business days. Rush options are available upon request at an additional cost.
Can you perform this test on a custom basis?
Absolutely! We offer customized services for all our clients. Whether it's adapting to specific customer requirements or performing additional analyses, we are committed to meeting your needs.
What is the turnaround time?
For standard ISO 9647 tests, our typical turnaround time is two business days. Rush options are available upon request at an additional cost.
What is the turnaround time?
For standard ISO 9647 tests, our typical turnaround time is two business days. Rush options are available upon request at an additional cost.
