EN 18801 Determination of Total Lipid Profile in Infant Formula
The determination of the total lipid profile in infant formula is a critical aspect of ensuring product safety and quality. This service strictly adheres to EN 18801:2015, which specifies methods for determining the total lipid content, fatty acid composition, and triglyceride profile of infant formulas. The test is essential because it helps manufacturers meet stringent regulatory requirements set forth by organizations such as the European Commission.
The total lipid profile includes various components like free fatty acids, monoacylglycerols, diacylglycerols, and triacylglycerols. These components play a crucial role in supporting infant growth and development. The test ensures that the formulated products meet specific nutritional requirements outlined by European Commission Regulation (EC) No 1252/2006. This regulation mandates the presence of certain essential fatty acids, such as linoleic acid (LA), alpha-linolenic acid (ALA), and docosahexaenoic acid (DHA).
The procedure involves several steps. Initially, a representative sample is obtained from the infant formula batch. Proper sampling ensures that the results are indicative of the entire lot's quality. The sample is then prepared according to standard procedures, which may include drying, grinding, and homogenization.
Once the sample preparation is complete, it undergoes extraction using solvents like chloroform-methanol mixture or hexane. This step aims to separate lipids from other components of the formula. After extraction, the lipid layer is isolated and analyzed for its fatty acid composition. Techniques such as gas chromatography (GC) and mass spectrometry (MS) are commonly used for this purpose.
The GC-MS analysis allows for precise identification and quantification of individual fatty acids. The results provide detailed information about the types and amounts of essential fatty acids present in the formula. This data is crucial for ensuring that the product meets nutritional guidelines set by regulatory bodies like the European Commission.
Another important part of the test involves assessing the triglyceride profile. Triglycerides are the primary form of stored fat in the body, and their presence in infant formulas must be within specified limits to ensure safety and efficacy. The testing process typically uses thin-layer chromatography (TLC) or high-performance liquid chromatography (HPLC). These methods help identify and quantify different types of triglycerides present in the formula.
The results obtained from these analyses are meticulously recorded and reported according to EN 18801. The report includes detailed information about the total lipid content, individual fatty acid profiles, and triglyceride compositions. This comprehensive data provides manufacturers with valuable insights into the nutritional quality of their products and helps them make informed decisions regarding formulation adjustments.
By adhering strictly to EN 18801, our laboratory ensures accurate and reliable testing results that comply with all relevant regulations. Our experienced team of analysts uses cutting-edge instrumentation and follows standardized protocols to deliver precise data. This level of accuracy is vital for maintaining product quality and ensuring compliance with international standards.
Applied Standards
| Standard | Description |
|---|---|
| EN 18801:2015 | Determination of total lipid profile in infant formula |
| European Commission Regulation (EC) No 1252/2006 | Nutritional requirements for infant formulas |
Benefits
- Ensures compliance with European Union regulations.
- Guarantees product safety and quality.
- Supports manufacturers in meeting nutritional guidelines.
- Provides detailed insights into the lipid profile of infant formulas.
- Facilitates adjustments to formulations based on test results.
The accurate determination of the total lipid profile is crucial for maintaining consistency and reliability in infant formula production. By adhering to EN 18801, manufacturers can be confident that their products meet all necessary regulatory requirements and provide optimal nutritional support for infants.
International Acceptance and Recognition
- The methods specified in EN 18801 are widely accepted across Europe.
- Results from this testing method are recognized by various international organizations.
- Compliance with this standard enhances the reputation of manufacturers globally.
- The results can be used for certification purposes.
The widespread acceptance and recognition of EN 18801 ensure that infant formulas tested in accordance with these standards are trusted both domestically and internationally. This is particularly important for companies exporting their products to different markets, as it simplifies compliance processes and builds consumer confidence.
