EN 18129 Determination of Carbohydrate Composition in Infant Cereals
The EN 18129 standard provides a comprehensive approach to determining the carbohydrate composition, particularly for sugars and other carbohydrates, in infant cereals. This method is critical for ensuring that products meet nutritional requirements set by regulatory authorities such as the European Commission (EC) and the World Health Organization (WHO).
The primary focus of this test is on providing accurate data about the carbohydrate content to support product labeling claims. Infant cereals are a staple in infant nutrition, and it's essential for manufacturers to ensure that the products meet stringent nutritional profiles.
According to EN 18129, carbohydrates play a crucial role in an infant’s diet as they provide energy and contribute to overall growth and development. The test aims at identifying various types of sugars including glucose, fructose, galactose, lactose, sucrose, maltose, and others that may be present. Additionally, it evaluates other carbohydrates such as starches and fiber.
The methodology outlined in EN 18129 involves several steps to ensure accurate results. First, a representative sample of the infant cereal is taken for analysis. Then, this sample undergoes extraction procedures designed to separate sugars from non-sugars components. Afterward, the isolated sugar fractions are analyzed using high-performance liquid chromatography (HPLC).
This process allows for precise quantification of individual sugars and other carbohydrates present in the product. The results obtained help manufacturers understand exactly what they are putting into their formulations and whether it aligns with recommended dietary guidelines.
Accurate carbohydrate composition analysis is vital not only from a nutritional standpoint but also because it influences the caloric content of the product, which impacts infant health directly. Misleading labeling could lead to improper feeding practices affecting an infant's growth trajectory negatively.
The standard specifies limits on certain sugars like sucrose and added fructose due to concerns about their potential impact on dental health in young children. By adhering strictly to these limits as per EN 18129, manufacturers can ensure their products are safe and beneficial for infants.
Scope and Methodology
| Sample Preparation | ||
| Sampling | Representative samples must be taken from the batch of infant cereals. | Ensure homogeneity by mixing thoroughly before sampling. |
| Extraction | Use appropriate solvents to extract sugars and other carbohydrates. | Follow manufacturer's instructions for optimal extraction efficiency. |
| Note: Always follow safety protocols when handling chemicals used in extraction processes. | ||
| Analytical Procedure | |
| Instrumentation | HPLC equipped with appropriate detectors for sugar and carbohydrate analysis. |
| Data Analysis | Analyze chromatograms to identify peaks corresponding to different sugars and carbohydrates. |
| Ensure accurate calibration of the HPLC system before starting analyses. | |
Why Choose This Test
Compliance with international standards: Ensures adherence to EC and WHO guidelines on infant nutrition.
Precision in labeling: Provides reliable data for accurate product labeling, enhancing consumer trust.
Risk management: Helps identify potential issues early, reducing risks associated with incorrect carbohydrate content.
Quality assurance: Ensures consistency across batches by providing repeatable test results.
Regulatory compliance: Avoids penalties and reputational damage from non-compliance.
Quality and Reliability Assurance
Use certified reference materials (CRMs) to calibrate instruments accurately.
Perform regular method validation studies to ensure precision and accuracy of results.
Adopt Good Laboratory Practices (GLP) to maintain high standards throughout the testing process.
Participate in proficiency testing programs offered by recognized bodies like APLA or ILAC to validate test methods.
