ISO 9233 Aflatoxin M1 Detection in Milk
The detection of aflatoxin M1 (AFM1) in milk is crucial for ensuring food safety and compliance with international standards. AFM1 is a potent carcinogen that can be metabolized from the mold toxin, aflatoxin B1, by rumen microorganisms in dairy animals like cows. This makes it essential to monitor its presence in raw and processed dairy products.
The ISO 9233 standard provides a standardized method for determining AFM1 in milk, which is widely recognized for its reliability and accuracy. By adhering to this protocol, laboratories can ensure that their test results are consistent with international best practices.
The testing process involves several critical steps: sample collection, extraction, purification, and quantification using immunoassay techniques or liquid chromatography-mass spectrometry (LC-MS/MS). These methods are chosen for their sensitivity and specificity in detecting even trace amounts of AFM1. The use of LC-MS/MS is particularly advantageous due to its high precision and ability to differentiate between closely related compounds.
Compliance with the ISO 9233 standard ensures that dairy products meet stringent quality control standards set by regulatory bodies worldwide, including the European Union (EU) and the United States Food and Drug Administration (FDA). This is especially important in markets where consumers have heightened awareness of food safety issues.
Our laboratory employs state-of-the-art instrumentation, such as automated liquid chromatographs with tandem mass spectrometers, to carry out this testing. These instruments provide accurate quantification results that are essential for regulatory compliance and consumer confidence. The process begins with the collection of milk samples from farms or processing facilities, followed by thorough preparation in our certified laboratories.
Once prepared, the samples undergo a series of purification steps designed to isolate AFM1 for measurement. This typically involves liquid-liquid extraction or solid-phase extraction methods, which are critical for ensuring accurate results. After purification, the sample is analyzed using LC-MS/MS, allowing us to detect even minute quantities of AFM1.
Our expertise lies not only in the technical aspects but also in providing comprehensive support throughout the testing process. From initial consultation on best practices and sampling techniques to detailed reporting and interpretation of results, our team ensures that clients receive reliable information they can trust. This level of service is particularly valuable for quality managers, compliance officers, and R&D engineers who need accurate data to make informed decisions.
By leveraging our extensive experience in food safety testing, we help ensure that dairy products meet stringent regulatory requirements set by international organizations like the Codex Alimentarius Commission. This commitment to excellence is reflected in our consistent adherence to ISO 9233 and other relevant standards.
| Standard Name | Year Published | Relevant Sections |
|---|---|---|
| ISO 9233:1990 | 1990 | Method for determining aflatoxin M1 in milk by immunoaffinity column cleanup followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) |
Applied Standards
The ISO 9233 standard is widely recognized for its reliability and accuracy in detecting aflatoxin M1 (AFM1) in milk. This standard provides a method for determining AFM1 using immunoaffinity column cleanup followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The process ensures that even trace amounts of AFM1 can be detected, making it an essential tool for ensuring food safety.
| Standard Name | Year Published | Relevant Sections |
|---|---|---|
| ISO 9233:1990 | 1990 | Method for determining aflatoxin M1 in milk by immunoaffinity column cleanup followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) |
Quality and Reliability Assurance
- Use of state-of-the-art LC-MS/MS instruments for accurate quantification.
- Compliance with ISO 9233:1990 standards throughout the testing process.
- Detailed documentation and traceability of all test procedures and results.
- Regular calibration and validation of equipment to ensure precision and accuracy.
International Acceptance and Recognition
The ISO 9233 standard is recognized globally for its reliability in detecting aflatoxin M1 (AFM1) in milk. Laboratories that adhere to this standard are trusted by regulatory bodies, food safety organizations, and consumers alike. The EU and FDA both accept results generated using the methods described in ISO 9233, ensuring harmonized testing across different regions.
Our laboratory’s strict adherence to these standards not only ensures compliance with international regulations but also builds trust among clients who rely on our expertise for accurate and reliable test results. This recognition is crucial for maintaining high-quality dairy products that meet the highest safety standards.
