ISO 15788 Conjugated Linoleic Acid Analysis in Dairy Products
The analysis of conjugated linoleic acid (CLA) under ISO 15788 is a critical process for ensuring the quality and safety of dairy products. CLA, a type of omega-6 fatty acid, has garnered significant attention due to its potential health benefits, including anti-carcinogenic properties and fat loss effects. This analysis helps in determining compliance with international standards that ensure the integrity of dairy product formulations.
Dairy products are essential components of balanced diets worldwide, and their quality is paramount for consumer safety and regulatory compliance. The presence of CLA can vary significantly based on the feed given to livestock and the processing methods employed during production. Accurate quantification of CLA content in dairy products is thus vital not only for nutritional labeling but also for verifying the efficacy of feed additives.
ISO 15788 provides a standardized method for the determination of cis-9, trans-11 (c9t11) and trans-10, cis-12 (t10c12) CLA isomers in dairy products. These specific isomers are the most widely studied due to their potential health impacts. The procedure involves extraction followed by chromatographic separation and detection using gas chromatography coupled with mass spectrometry (GC-MS). This method ensures precision and accuracy, which are essential for maintaining product consistency.
The significance of this analysis extends beyond just compliance with international standards. It also aids in quality control processes within dairy processing plants. By regularly monitoring CLA levels, companies can ensure that their products meet not only regulatory requirements but also consumer expectations regarding health benefits and nutritional content. This service is particularly valuable for firms involved in organic or specialty milk production.
The analytical procedure under ISO 15788 involves several steps: sample preparation, extraction, purification, derivatization, and finally, analysis via GC-MS. Each step requires meticulous attention to detail to avoid errors that could lead to inaccurate results. Sample preparation typically includes homogenizing the dairy product to ensure a representative sample is analyzed. Extraction methods often use solvents like methanol or chloroform, followed by purification steps such as column chromatography.
Derivatization with reagents like methoxyamine hydrochloride and N-methyl-N-trimethylsilyl trifluoroacetamide enhances the detection sensitivity of CLA isomers in GC-MS. The chromatographic separation ensures that individual CLA isomers are well-resolved, allowing for precise quantification. Once analyzed, the data is interpreted to provide concentrations of each isomer within specified limits.
The importance of this service cannot be overstated, especially given the growing consumer interest in organic and specialty dairy products. Compliance with ISO 15788 helps manufacturers maintain trust among their customer base by ensuring product quality meets rigorous international standards. Moreover, it supports ongoing research into the health benefits of CLA, potentially leading to new product innovations.
In summary, the analysis of conjugated linoleic acid (CLA) in dairy products under ISO 15788 is a cornerstone for maintaining product integrity and regulatory compliance. It ensures that dairy products not only meet international standards but also provide consistent health benefits as intended by manufacturers.
Applied Standards
The analysis of conjugated linoleic acid (CLA) in dairy products under ISO 15788 is based on the International Organization for Standardization's standard, ISO 15788:2013. This standard provides a detailed procedure for determining cis-9, trans-11 (c9t11) and trans-10, cis-12 (t10c12) CLA isomers in milk, cream, butter, cheese, and other dairy products.
The standard specifies the sample preparation methods, extraction techniques, purification procedures, derivatization steps, and final analytical methods using gas chromatography coupled with mass spectrometry (GC-MS). Compliance with this standard ensures accuracy and consistency in CLA analysis across different laboratories and industries.
Industry Applications
| Dairy Product | Milk |
|---|---|
| Type of Analysis | Conjugated Linoleic Acid (CLA) Analysis |
| Target Isomers | c9t11 and t10c12 CLA isomers |
- Dairy product manufacturers
- R&D departments focusing on organic dairy products
- Regulatory bodies ensuring compliance with international standards
- Ensuring product quality and safety
- Supporting nutritional labeling requirements
- Aiding in the development of new, CLA-enriched specialty dairy products
The analysis under ISO 15788 plays a crucial role in these applications by providing accurate and reliable data on CLA isomer concentrations. This information is vital for maintaining product consistency and ensuring that dairy products meet both regulatory requirements and consumer expectations.
Eurolab Advantages
At Eurolab, we offer comprehensive services for the analysis of conjugated linoleic acid (CLA) in dairy products under ISO 15788. Our expertise ensures that our clients receive accurate and reliable results every time.
- State-of-the-art Laboratory Facilities: Equipped with advanced GC-MS instruments, we provide precise and consistent analytical results.
- Trained Professionals: Our team of experts is well-trained in the latest techniques and procedures for CLA analysis.
- Comprehensive Reporting: We offer detailed reports that include raw data, interpretation, and compliance status with international standards.
Our commitment to excellence ensures that every client receives the highest quality service. Whether you are a dairy product manufacturer or an R&D engineer, Eurolab is your trusted partner for accurate CLA analysis.
