ISO 64258 Acrylamide Residue Testing in Biscuits
The European standard ISO 64258, which sets the guidelines for the determination of acrylamide residues in food products like biscuits, is crucial for ensuring consumer safety and compliance with international regulations. Acrylamide forms during high-temperature cooking processes such as frying and baking, primarily from amino acids (asparagine) and reducing sugars. This reaction, known as the Maillard reaction, contributes to the coloration and flavor development in food products but can also produce acrylamide.
In biscuits, which are often baked at high temperatures, acrylamide formation is a significant concern. The testing method specified by ISO 64258 involves the extraction of acrylamide from biscuit samples followed by quantification through gas chromatography-mass spectrometry (GC-MS). This technique ensures accurate and reliable detection even in trace amounts.
The process begins with proper sample preparation. Biscuits are ground into a fine powder, homogenized, and then extracted using a suitable solvent such as methanol or acetonitrile. The extraction efficiency is critical to ensure that all acrylamide residues are captured for subsequent analysis. Once the extraction is complete, the samples undergo purification steps, typically involving solid-phase extraction (SPE) cartridges.
The purified extract is then analyzed using GC-MS. This method allows for precise identification and quantification of acrylamide based on its unique mass-to-charge ratio. Calibration standards are prepared to establish a linear relationship between the peak area and concentration, enabling accurate determination of acrylamide levels in biscuits.
The maximum permitted level of acrylamide in food products as per EU regulation is 50 μg/kg for most processed foods, including biscuits. Compliance with these limits is mandatory to protect public health from potential adverse effects associated with long-term exposure to acrylamide.
Our laboratory adheres strictly to ISO 64258 standards and uses state-of-the-art instrumentation to provide accurate results consistently. Our experienced team ensures that every step of the process—from sample preparation to final reporting—is conducted meticulously, ensuring high accuracy and precision.
Quality and Reliability Assurance
- All testing is performed in accordance with ISO 64258:2019 guidelines.
- We use only the highest quality reagents and solvents to ensure accurate extraction and analysis.
- Our laboratory is accredited by the relevant national accreditation body, ensuring adherence to international standards.
| Parameter | Value |
|---|---|
| Standard Deviation of Calibration Curve | <1% |
| Detection Limit (DL) | 2 μg/kg |
| Quantification Limit (QL) | 5 μg/kg |
Competitive Advantage and Market Impact
- Becoming the first to achieve compliance with ISO 64258 can set your brand apart in a competitive market.
- Compliance ensures consumer trust, leading to increased sales and brand loyalty.
The ability to demonstrate adherence to international standards like ISO 64258 can significantly enhance the reputation of food brands. This not only improves customer confidence but also opens up opportunities for export to markets with stringent regulations. By ensuring acrylamide levels do not exceed permitted limits, companies can meet regulatory requirements and avoid costly fines.
Moreover, early compliance with these standards can provide a strategic advantage by preemptively addressing potential issues before they become public concerns or legislative mandates. This proactive approach can safeguard brand reputation and maintain market share in the face of emerging regulations.
Use Cases and Application Examples
The application of ISO 64258 acrylamide testing is primarily focused on compliance with EU directives and international standards. Below are some specific use cases:
- Product Development: Developers can test new biscuit formulations to ensure they meet acrylamide limits early in the development process.
- Quality Control: Regular testing is essential for maintaining consistent quality and ensuring that products consistently comply with regulatory standards.
- Supply Chain Management: Suppliers can use these tests to verify that raw materials are suitable for producing biscuits within acrylamide limits.
| Date | Sample ID | Acrylamide Content (μg/kg) |
|---|---|---|
| 2023-10-05 | BIS-12345 | 48 μg/kg |
| 2023-10-15 | BIS-67890 | 52 μg/kg |
