ISO 23989 Vitamin B1 (Thiamine) Analysis in Feed Supplements
The ISO 23989 standard provides a robust framework for the quantitative determination of thiamine (Vitamin B1) content in feed supplements. This service is essential for ensuring compliance with international standards and regulatory requirements, which help maintain the quality, safety, and efficacy of dietary supplements intended for animal consumption.
Thiamine plays a critical role in energy metabolism, nerve function, and carbohydrate metabolism in animals. Ensuring accurate levels of thiamine in feed supplements is crucial to prevent deficiencies that could lead to poor growth performance, reproductive issues, and even neurological disorders. This analysis supports the development of high-quality feed formulations tailored to specific animal species and life stages.
The analytical method outlined in ISO 23989 involves a combination of sample preparation, extraction techniques, and chromatographic separation followed by quantification using UV-visible spectroscopy or HPLC (High-Performance Liquid Chromatography). This approach ensures precise measurement and reliable results that are repeatable and reproducible.
Sample preparation is the first step in this process. The feed supplement sample is dried to a constant weight, ground to a fine powder, and then accurately weighed into a beaker. An extraction solvent (such as acetonitrile or methanol) is added, and the mixture is shaken thoroughly before filtration through a 0.45-µm filter. The filtrate is then concentrated under reduced pressure if necessary.
For analysis, an aliquot of the extract is injected into the chromatographic system for separation based on retention time. Detection occurs at a wavelength of 260 nm (UV-visible spectroscopy) or using a photodiode array detector in HPLC systems. The peak area corresponding to thiamine is used to calculate its concentration according to calibration standards prepared previously.
The accuracy and precision of the results depend heavily on proper sample handling, extraction efficiency, and chromatographic conditions. Therefore, adherence to strict procedural guidelines as specified in ISO 23989 ensures reliable outcomes that can be trusted for regulatory submissions or internal quality control purposes.
Compliance with this standard is particularly important given the increasing demand for organic and sustainable feed ingredients worldwide. By accurately measuring thiamine content, laboratories using this method contribute to more responsible agricultural practices by avoiding over- or under-supplementation of essential nutrients in animal diets.
In summary, ISO 23989 offers a precise analytical technique for determining thiamine levels in feed supplements, which is vital for maintaining the health and productivity of livestock. This service supports both regulatory compliance and good manufacturing practices (GMP), ensuring that products meet stringent quality standards set forth by international bodies.
Why It Matters
Accurate measurement of thiamine content in feed supplements is crucial for several reasons:
- To prevent deficiencies leading to poor animal health and performance.
- To ensure compliance with regulatory requirements set by various countries around the world.
- To support responsible agricultural practices through appropriate nutrient supplementation.
By adhering to ISO 23989, laboratories play a key role in maintaining the integrity of feed products, thereby contributing to sustainable food production systems.
Why Choose This Test
- Accurate quantification: The method provides precise measurements, essential for regulatory compliance and quality assurance.
- International recognition: Results are accepted worldwide due to its adherence to ISO standards.
- Scientific reliability: Proven methodology backed by extensive research and validation.
The combination of these factors makes the ISO 23989 analysis a preferred choice for laboratories aiming to provide reliable, accurate data on thiamine content in feed supplements.
Use Cases and Application Examples
| Application Scenario | Description |
|---|---|
| Quality Control | Detecting variations in thiamine content across batches to ensure consistency. |
| Development of New Formulations | Optimizing vitamin supplementation levels based on analytical results. |
| Compliance Monitoring | Verifying that feed products meet regulatory requirements for thiamine content. |
| R&D | Evaluating the effectiveness of different feed ingredients in enhancing thiamine availability to animals. |
| Supply Chain Audits | Assessing supplier quality by verifying reported thiamine levels against actual measurements. |
| Consumer Safety | Ensuring that feed products do not contain excessive amounts of thiamine which could pose health risks to animals. |
The diverse applications of this analysis highlight its importance in various stages of the feed production process, from formulation and quality control to regulatory compliance and research development.
