EN 13804 Vitamin E Residue Analysis in Pet Food
The European standard EN 13804 outlines the procedure for analyzing vitamin E residues in pet food. This method is crucial for ensuring that pet food products comply with regulatory limits set to protect both pets and humans from potential health risks associated with excessive or toxic levels of vitamin E.
Vitamin E, also known as tocopherol, plays a vital role in maintaining the overall health of pets by acting as an antioxidant. However, when consumed in excess, it can lead to toxicity symptoms such as muscle weakness and liver damage. Therefore, accurate residue analysis is essential for ensuring that pet food meets safety standards.
The procedure described in EN 13804 involves a series of steps aimed at extracting vitamin E from the sample matrix, followed by its quantification using high-performance liquid chromatography (HPLC) with UV detection. The extraction process typically uses solvents like acetonitrile and water to dissolve fats and other components that interfere with the analytical procedure.
The standard specifies detailed instructions for preparing samples, including homogenization, filtration, and dilution if necessary. After extraction, the sample is analyzed using a validated HPLC method that ensures reliable detection of vitamin E residues at trace levels.
Compliance with this standard is mandatory for manufacturers to ensure their products meet regulatory requirements. Regulatory authorities use these results to verify compliance during inspections and audits. In some cases, consumers may also request independent third-party testing to build trust in the product's safety and quality.
The importance of accurate residue analysis extends beyond mere compliance; it helps maintain consumer confidence by ensuring that pet food products are safe for consumption. Regulatory authorities worldwide recognize standards like EN 13804 as authoritative guidelines, making adherence essential for global market access.
| Step | Description |
|---|---|
| Sample Preparation | Homogenize, filter, and dilute samples as needed. |
| Extraction | Use solvents like acetonitrile and water to extract vitamin E residues. |
| HPLC Analysis | Analyze the extracted samples using validated HPLC methods. |
Industry Applications
The EN 13804 standard finds application in various sectors of the pet food industry, including manufacturing, quality control, and regulatory compliance. Manufacturers rely on this method to ensure their products meet safety standards before they reach the market.
- Manufacturers: To ensure product safety and compliance with regulations.
- Quality Control Departments: For consistent monitoring of vitamin E levels in pet food formulations.
- Regulatory Authorities: To verify compliance during inspections and audits.
- R&D Engineers: To develop new formulas that adhere to regulatory limits while maintaining nutritional value.
The table below highlights the key steps involved in the analysis of vitamin E residues in pet food:
Eurolab Advantages
At Eurolab, we provide comprehensive and reliable EN 13804 compliance services for vitamin E residue analysis in pet food. Our experienced team of scientists and technicians ensures that every step of the process adheres strictly to the standard's requirements.
- Accurate Results: Using state-of-the-art HPLC equipment, we deliver precise results that meet or exceed regulatory standards.
- Comprehensive Support: Our team offers guidance on sample preparation and troubleshooting issues that may arise during analysis.
- Expertise: With years of experience in pet food testing, our scientists stay updated with the latest methodologies and best practices.
- Rapid Turnaround: We understand the importance of timely results for your business operations. Our fast turnaround times ensure minimal disruption to your workflow.
Quality and Reliability Assurance
We take pride in our commitment to quality and reliability, ensuring that every analysis conducted at Eurolab meets the highest standards. Our services are backed by rigorous quality assurance protocols aimed at delivering consistent and accurate results.
- ISO/IEC 17025: Our laboratory is fully accredited under this international standard for testing laboratories, guaranteeing our proficiency in technical competence.
- Validation: All analytical methods are validated to ensure they meet the required precision and accuracy standards.
- Continuous Improvement: We continuously review and update our procedures to align with the latest industry best practices and regulatory requirements.
