EN 13104 Vitamin D3 Quantification in Animal Premixes
The quantification of vitamin D3 (cholecalciferol) in animal premixes is crucial for ensuring the optimal nutritional content necessary to support healthy growth and development in animals. This procedure, specified by EN 13104:2006, provides a standardized approach to accurately quantify this essential nutrient in premixes used in the formulation of feed for various animal species.
The vitamin D3 quantification test is particularly important because it guarantees that animals receive an adequate supply of this fat-soluble vitamin. Vitamin D3 plays a vital role in calcium absorption, bone mineralization, and immune function. Deficiencies or excesses can lead to severe health issues such as rickets in young animals or osteomalacia in adults.
The standard method involves the analysis of samples using High-Performance Liquid Chromatography (HPLC). The process begins with the extraction of vitamin D3 from the premix sample, which is then purified and quantified. The HPLC technique allows for precise measurement by separating compounds based on their retention time.
Accurate quantification ensures that feed manufacturers comply with regulatory requirements set forth by various national and international standards bodies. This service helps in maintaining product consistency and quality while also ensuring adherence to nutritional guidelines. For instance, the European Union has specific recommendations regarding vitamin D3 levels in animal feeds, which this test supports.
The EN 13104 standard specifies that the quantification method should be validated for accuracy, precision, and linearity. This validation ensures that the results obtained are reliable and reproducible across different laboratories. The standard also mandates the use of certified reference materials (CRMs) to calibrate the analytical instruments.
The importance of this test cannot be overstated in the context of animal husbandry and nutrition. By ensuring accurate quantification, feed manufacturers can optimize their formulations, thereby enhancing productivity and health outcomes for livestock. This, in turn, supports sustainable agricultural practices by minimizing waste and maximizing resource efficiency.
Moreover, compliance with EN 13104 is crucial for maintaining market access to international markets that have stringent quality control measures in place. Many countries require proof of conformity to such standards as part of the importation process. This ensures that feed products meet not only local but also global regulatory requirements.
The quantification method outlined in EN 13104 is suitable for a wide range of animal species, including poultry, swine, and dairy cows. The variability in dietary needs across different species underscores the importance of accurate vitamin D3 quantification to tailor feeds appropriately.
Applied Standards
Standard Reference | EN 13104:2006 |
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Title | Vitamin D3 in animal premixes (high-performance liquid chromatography) |
Publisher | European Committee for Standardization (CEN) |
Type of Standard | Technical Specification |
Date of Publication | 2006-12 |
This standard provides a detailed protocol for the quantification of vitamin D3 in animal premixes using HPLC. It includes specific instructions on sample preparation, calibration procedures, and method validation to ensure accurate results.
The use of this standard ensures that laboratories conducting such analyses adhere to internationally recognized protocols, thereby enhancing the reliability and consistency of test outcomes. Compliance with EN 13104 is essential for labs aiming to provide accurate and reliable quantification services in line with global best practices.
Why Choose This Test?
The EN 13104 method is internationally recognized, ensuring consistent results across different laboratories and jurisdictions.
This test guarantees compliance with international regulations regarding vitamin D3 levels in animal feeds.
It supports sustainable agricultural practices by optimizing feed formulations for maximum efficiency and minimal waste.
The use of this standardized method ensures that the nutritional content of premixes is accurately quantified, leading to healthier livestock and improved productivity. This, in turn, has positive implications for both animal health and environmental sustainability.
Accurate vitamin D3 quantification also helps in reducing the risk of deficiencies or excesses, which can have detrimental effects on animals' health and welfare.
Customer Impact and Satisfaction
Ensures compliance with international standards, enhancing market access for feed manufacturers.
Supports the development of high-quality animal feeds that meet nutritional requirements precisely.
Maintains product consistency and quality, leading to satisfied customers in both domestic and export markets.
The accurate quantification of vitamin D3 not only meets regulatory requirements but also enhances consumer confidence by ensuring the safety and efficacy of animal feeds. This service plays a critical role in supporting the goals of feed manufacturers and contributing positively to the broader agricultural sector.
Customers benefit from this test through improved product quality, enhanced nutritional profiles, and better management of costs associated with feed formulation. The precision provided by EN 13104 ensures that feeds are optimized for performance, thereby contributing to increased productivity and profitability in livestock farming.