EN ISO 19020 Detection of Yersinia enterocolitica in Minced Meat by Real-Time PCR
The detection and quantification of pathogens such as Yersinia enterocolitica in food products is crucial for ensuring the safety and quality of meat products. This service adheres to the European Standard EN ISO 19020, which outlines a precise method for detecting and quantifying this pathogen through real-time polymerase chain reaction (PCR) technology.
The process starts with the collection of minced meat samples, followed by their homogenization and dilution. DNA extraction is then performed using standard methods compatible with real-time PCR. The extracted DNA undergoes a series of amplification steps to detect specific genetic sequences associated with Yersinia enterocolitica. This method allows for the accurate identification of this pathogen, which can cause gastrointestinal and systemic infections in humans.
The real-time PCR technology used ensures high sensitivity and specificity. The reaction mixture contains primers and probes designed to target unique regions of the Yersinia enterocolitica genome. During amplification, fluorescence signals are generated, which are quantified in real-time to determine the presence and concentration of the pathogen.
The method is validated by comparing results with traditional culture-based methods where appropriate. This ensures that the PCR-based approach provides accurate and reliable data. The service also includes a comprehensive report that details the test procedure, raw data, interpretation, and conclusions regarding the presence or absence of Yersinia enterocolitica.
The use of this standardized method is essential for ensuring compliance with food safety regulations across Europe. It provides quality assurance to meat producers, importers, distributors, and retailers who are committed to maintaining high standards in their supply chains.
For accurate detection, the correct handling of samples is crucial. Samples should be collected aseptically, stored at appropriate temperatures, and processed as soon as possible after collection. This ensures that the integrity of the DNA extracted from the pathogen remains intact, leading to more reliable results.
Industry Applications
| Application Area | Description |
|---|---|
| Meat Processing Plants | Ensure the safety and quality of minced meat products by detecting Yersinia enterocolitica. |
| Food Safety Authorities | Support regulatory compliance with international standards for food safety. |
| Distribution and Retail Chains | Assure the quality of products reaching consumers, protecting public health. |
International Acceptance and Recognition
- The method adheres to the European Standard EN ISO 19020, ensuring international recognition.
- This standard is widely accepted in Europe for its accuracy and reliability.
- The results obtained from this service are internationally recognized and can be used as a basis for regulatory compliance.
Competitive Advantage and Market Impact
- Enhances brand reputation by ensuring product safety and quality.
- Safeguards against potential recalls or legal issues related to foodborne pathogens.
- Aids in maintaining a compliant supply chain, which is crucial for long-term business success.
