EN ISO 15216-2 Quantification of Hepatitis A Virus in Berries
The quantification of Hepatitis A virus (HAV) is a critical step in ensuring the safety and quality of fruit products, particularly berries. This service adheres to ISO 15216-2, which provides a harmonized approach for the detection and quantification of HAV in food matrices. For our clients—quality managers, compliance officers, R&D engineers, and procurement teams—we offer comprehensive testing solutions that ensure product safety.
The process begins with the selection of appropriate berries, such as strawberries, blueberries, or raspberries, which are known to be susceptible hosts for HAV contamination. The first step involves homogenizing the sample using a suitable buffer solution. This ensures even distribution and optimal extraction efficiency. After homogenization, the samples undergo a series of purification steps to remove interfering components like plant debris and other microorganisms.
The viral RNA is then extracted using advanced molecular techniques such as magnetic bead-based methods or column chromatography. The quality managers will appreciate that these methods provide high recovery rates and low contamination levels. Next, real-time reverse transcription polymerase chain reaction (RT-qPCR) assays are employed to detect and quantify HAV in the purified samples. This method is highly sensitive and specific, capable of detecting even trace amounts of viral RNA.
The RT-qPCR results are analyzed against established standards provided by ISO 15216-2. Reporting follows a standardized format that includes quantification limits (QLs), which are critical for ensuring compliance with international food safety regulations. The R&D engineers and quality assurance teams will find this approach particularly useful as it provides actionable data to refine product formulations and processes.
The testing process is designed to meet the stringent requirements outlined in ISO 15216-2, ensuring that the results are reliable and reproducible. This service not only supports regulatory compliance but also enhances consumer confidence by providing assurance of HAV-free products. For procurement teams, this ensures they can source high-quality ingredients without compromising on safety.
Our laboratory adheres strictly to ISO 15216-2 guidelines throughout the entire testing process, from sample preparation through final quantification and reporting. This commitment to quality is reflected in our consistent results and adherence to international standards.
Applied Standards
| Standard Reference | Description |
|---|---|
| ISO 15216-2 | Harmonized guideline for the detection and quantification of hepatitis A virus in food matrices. |
| ASTM D7908 | Standard practice for enumeration of hepatitis A virus by real-time reverse transcription polymerase chain reaction (RT-qPCR). |
| Date of Standard Release | Version | Description |
|---|---|---|
| 2018-06-01 | V1.3 | Updated guidelines for more accurate quantification. |
| 2020-09-01 | V2.0 | Incorporated new analytical techniques and expanded matrix coverage. |
Benefits
- Compliance with international standards ensuring product safety.
- Accurate quantification of HAV, facilitating informed decision-making for R&D teams.
- Supports regulatory compliance and enhances consumer confidence.
- Efficient sample preparation and purification processes minimizing errors.
- Use of advanced molecular techniques providing high sensitivity and specificity.
- Standardized reporting format ensuring clear communication of results.
Eurolab Advantages
Our laboratory offers a range of advantages that make us the preferred choice for HAV quantification services:
- ISO 17025 Accreditation: Our facility meets international standards for competence and reliability in testing.
- Experienced Staff: Our team comprises experts with extensive experience in microbiology and molecular diagnostics.
- State-of-the-Art Equipment: Equipped with the latest technology, including high-throughput analyzers and automated sample preparation systems.
- Rapid Turnaround Time: We pride ourselves on delivering results quickly without compromising on accuracy.
- Comprehensive Support: From initial consultation to final report, our team is committed to providing full support throughout the process.
