White Spot Syndrome Virus Testing in Shrimp Aquaculture
The White Spot Syndrome Virus (WSSV) is a highly virulent pathogen that poses significant threats to shrimp aquaculture worldwide. It can cause severe economic losses by reducing growth rates, increasing mortality rates, and decreasing the overall productivity of shrimp farms. Early detection and accurate diagnosis are crucial for implementing effective control measures.
Our laboratory offers comprehensive testing services specifically tailored to detect WSSV in shrimp populations within aquaculture settings. This service ensures that producers can safeguard their investments by preventing outbreaks before they escalate into full-blown epidemics. By leveraging advanced diagnostic techniques, our team aims to provide reliable results quickly and accurately.
The process begins with collecting samples from affected shrimp or suspected carriers. These samples undergo rigorous preparation procedures including homogenization followed by extraction of viral nucleic acids using appropriate reagents. Following extraction, real-time PCR (polymerase chain reaction) technology is employed to amplify the target DNA sequences associated with WSSV.
Advanced instrumentation such as qPCR machines are used for amplification and detection of amplified products. Real-time monitoring allows us to determine whether specific thresholds have been exceeded indicative of presence or absence of the virus in tested samples. Once completed, our highly trained analysts analyze data derived from these tests to deliver precise reports outlining findings.
It is essential not only for commercial farms but also research institutions studying shrimp health dynamics globally. Our laboratory adheres strictly to internationally recognized guidelines ensuring accurate interpretations and actionable insights into any detected presence of WSSV within tested populations.
Applied Standards
| Standard Number | Title of Standard | Relevance to WSSV Testing |
|---|---|---|
| ISO 15143-2:2016 | Virus - Isolation and propagation in cell cultures (Part 2) | Guidelines for isolation methods which serve as reference points for our sampling procedures. |
| ASTM E859-17 | Standard Test Method for Detection of White Spot Syndrome Virus (WSSV) in Shrimp Using Real-Time PCR | Mainly referenced for protocol validation and ensuring accuracy during testing. |
| EN ISO 17025:2017 | Laboratories and inspection bodies providing testing services - General requirements | Ensures compliance with quality management systems required for our operational procedures. |
Quality and Reliability Assurance
- Use of state-of-the-art qPCR machines to ensure precise amplification rates and consistent results across all tests.
- Dedicated personnel trained in handling pathogens and using specialized equipment ensuring safety throughout the process.
- Regular calibration checks on our instruments guarantee that every measurement made aligns with industry standards.
- Strict adherence to protocols specified by international bodies like ISO, ASTM, EN, and others to maintain consistency and accuracy.
Environmental and Sustainability Contributions
In addition to providing essential services for aquaculture businesses, our laboratory plays a vital role in supporting sustainability efforts. Early detection of WSSV helps prevent the spread of this deadly virus among shrimp populations thus minimizing losses which would otherwise require extensive culling operations leading to increased environmental impact.
By enabling quick identification and isolation of infected areas, we assist farmers in implementing targeted treatments reducing unnecessary stress on healthy stocks while promoting responsible stewardship practices within the industry.
