USDA MLG 25.01 Rapid PCR Screening for Foodborne Viruses in Dairy
The USDA MLG 25.01 standard provides a rapid polymerase chain reaction (PCR) screening method to detect foodborne viruses in dairy products. This testing protocol is essential for ensuring the safety and quality of milk, cheese, yogurt, and other dairy products consumed by humans. The test focuses on detecting Norovirus and Rotavirus, two common foodborne pathogens that can cause significant gastrointestinal distress.
The USDA MLG 25.01 method utilizes advanced PCR technology to amplify specific segments of viral RNA or DNA. This allows for the identification of these viruses at extremely low concentrations, making it possible to detect them even in complex dairy matrices. The assay is particularly useful for early detection and containment strategies, helping food processors avoid costly recalls and potential public health crises.
The testing process begins with sample collection from the dairy facility or product batch. Samples are typically collected from raw milk, finished products, and environmental surfaces. Once collected, specimens undergo a series of preparatory steps, including homogenization, filtration, and RNA/DNA extraction to ensure that any viral particles are isolated for accurate PCR analysis.
The actual PCR reaction is performed using a real-time PCR machine, which monitors the amplification process in real time. This allows for precise quantitation and differentiation between target viruses and potential non-specific amplifications. The assay typically targets specific regions of the norovirus and rotavirus genomes to ensure specificity and accuracy.
The results from the PCR test are reported within a few hours, providing rapid feedback on the presence or absence of these viruses in the dairy product. This enables quick corrective actions such as process adjustments, ingredient rejections, or batch isolation if contamination is detected. The report also includes detailed information about the detection method, sample preparation steps, and any relevant performance criteria.
The USDA MLG 25.01 standard ensures that all tests are conducted under controlled conditions to minimize the risk of false positives or negatives. This is critical in maintaining consumer confidence and regulatory compliance with food safety regulations.
For quality managers, compliance officers, R&D engineers, and procurement personnel, this rapid screening method offers a robust tool for ensuring product integrity and safety. By implementing USDA MLG 25.01, dairy processors can protect their brand reputation and ensure that consumers are provided with safe food products.
Applied Standards
| Standard |
Description |
| USDA MLG 25.01 |
Rapid PCR screening for foodborne viruses in dairy products, focusing on Norovirus and Rotavirus. |
| ISO 6873:2019 |
Methods of sampling milk and milk products. |
| Standard |
Description |
| ASTM D5837-20 |
Determination of total coliforms in water, milk, and dairy products by membrane filtration. |
| EN ISO 14695:2003 |
Animal derived foods - Determination of noroviruses and sapoviruses in shellfish and other animal derived foodstuffs. |
EuroLab Advantages
At EuroLab, we pride ourselves on providing the most accurate and reliable testing services in the industry. Our state-of-the-art facilities are equipped with cutting-edge PCR machines that ensure consistent results every time. Our team of highly trained professionals guarantees that each sample is handled with utmost care, from initial collection to final analysis.
We offer quick turnaround times for our tests, ensuring that you receive your results as soon as possible. This allows for swift decision-making and timely corrective actions if necessary. Additionally, we provide comprehensive reports detailing the entire testing process, including sample preparation, assay conditions, and detection criteria.
EuroLab is committed to maintaining high standards of quality and reliability in all our services. Our adherence to international standards such as USDA MLG 25.01 ensures that you receive results that are both accurate and consistent with industry best practices.
Why Choose This Test?
- Rapid detection of Norovirus and Rotavirus in dairy products.
- Accurate PCR technology for precise quantitation and differentiation between target viruses.
- Comprehensive reporting including sample preparation steps, assay conditions, and performance criteria.
- Quick turnaround times to enable swift corrective actions if contamination is detected.
- Highly trained professionals ensuring meticulous handling of samples from initial collection to final analysis.
Frequently Asked Questions
What is the turnaround time for USDA MLG 25.01 testing?
We typically provide results within 24-48 hours after sample receipt, ensuring rapid feedback.
Can this test detect all types of foodborne viruses in dairy?
The assay is specifically designed to target Norovirus and Rotavirus. Detection of other viruses may require different methods.
Is this test suitable for all types of dairy products?
Yes, the assay is applicable to a wide range of dairy products including milk, cheese, yogurt, and other processed items.
How does this test ensure accuracy and reliability?
We use state-of-the-art PCR machines and highly trained professionals to handle each sample meticulously from initial collection to final analysis.
What is the cost of USDA MLG 25.01 testing?
The cost varies depending on the volume and complexity of the samples, but we offer competitive rates to ensure affordability.
Can you provide a detailed report after each test?
Absolutely. Each report includes comprehensive details about sample preparation, assay conditions, and detection criteria.
How long does it take to prepare samples for testing?
Sample preparation typically takes approximately 2-3 hours, after which the actual PCR reaction can be initiated.
What should I do if contamination is detected in my dairy products?
We recommend immediate corrective actions such as process adjustments, ingredient rejections, or batch isolation. Our team can assist with implementation.
Is this test suitable for all types of dairy products?
Yes, the assay is applicable to a wide range of dairy products including milk, cheese, yogurt, and other processed items.
How does this test ensure accuracy and reliability?
We use state-of-the-art PCR machines and highly trained professionals to handle each sample meticulously from initial collection to final analysis.
What is the cost of USDA MLG 25.01 testing?
The cost varies depending on the volume and complexity of the samples, but we offer competitive rates to ensure affordability.
Can you provide a detailed report after each test?
Absolutely. Each report includes comprehensive details about sample preparation, assay conditions, and detection criteria.
How long does it take to prepare samples for testing?
Sample preparation typically takes approximately 2-3 hours, after which the actual PCR reaction can be initiated.
What should I do if contamination is detected in my dairy products?
We recommend immediate corrective actions such as process adjustments, ingredient rejections, or batch isolation. Our team can assist with implementation.
How does this test ensure accuracy and reliability?
We use state-of-the-art PCR machines and highly trained professionals to handle each sample meticulously from initial collection to final analysis.
What is the cost of USDA MLG 25.01 testing?
The cost varies depending on the volume and complexity of the samples, but we offer competitive rates to ensure affordability.
Can you provide a detailed report after each test?
Absolutely. Each report includes comprehensive details about sample preparation, assay conditions, and detection criteria.
How long does it take to prepare samples for testing?
Sample preparation typically takes approximately 2-3 hours, after which the actual PCR reaction can be initiated.
What should I do if contamination is detected in my dairy products?
We recommend immediate corrective actions such as process adjustments, ingredient rejections, or batch isolation. Our team can assist with implementation.
What is the cost of USDA MLG 25.01 testing?
The cost varies depending on the volume and complexity of the samples, but we offer competitive rates to ensure affordability.
Can you provide a detailed report after each test?
Absolutely. Each report includes comprehensive details about sample preparation, assay conditions, and detection criteria.
How long does it take to prepare samples for testing?
Sample preparation typically takes approximately 2-3 hours, after which the actual PCR reaction can be initiated.
What should I do if contamination is detected in my dairy products?
We recommend immediate corrective actions such as process adjustments, ingredient rejections, or batch isolation. Our team can assist with implementation.
Can you provide a detailed report after each test?
Absolutely. Each report includes comprehensive details about sample preparation, assay conditions, and detection criteria.
How long does it take to prepare samples for testing?
Sample preparation typically takes approximately 2-3 hours, after which the actual PCR reaction can be initiated.
What should I do if contamination is detected in my dairy products?
We recommend immediate corrective actions such as process adjustments, ingredient rejections, or batch isolation. Our team can assist with implementation.
How long does it take to prepare samples for testing?
Sample preparation typically takes approximately 2-3 hours, after which the actual PCR reaction can be initiated.
What should I do if contamination is detected in my dairy products?
We recommend immediate corrective actions such as process adjustments, ingredient rejections, or batch isolation. Our team can assist with implementation.
What should I do if contamination is detected in my dairy products?
We recommend immediate corrective actions such as process adjustments, ingredient rejections, or batch isolation. Our team can assist with implementation.
