ISO 15216-2 Quantification of Norovirus in Bivalve Mollusks by Real-Time PCR
The quantification of noroviruses, particularly in bivalve mollusks such as clams and oysters, is crucial for ensuring food safety. Noroviruses are the leading cause of non-bacterial gastroenteritis worldwide, responsible for millions of cases annually. These viruses are known to persist on surfaces and within contaminated food products, making accurate quantification a priority.
The ISO 15216-2 standard provides guidelines for quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) methods used in the detection and quantification of noroviruses. This method allows for precise measurement of viral load, which is essential for regulatory compliance and to protect public health.
Our laboratory employs state-of-the-art equipment and adheres strictly to ISO 15216-2 guidelines to ensure reliable results. Our team of experts meticulously prepares samples, conducts qRT-PCR analysis, and interprets data to provide accurate quantification reports. This service is vital for food producers, retailers, and regulatory bodies aiming to maintain high standards of food safety.
The process begins with the collection and preservation of bivalve mollusk samples. Samples are then prepared in a controlled environment following strict protocols to prevent contamination. Once prepared, they undergo qRT-PCR analysis using optimized reagents and primers specific for norovirus detection. This ensures high specificity and sensitivity, which is critical for accurate quantification.
Our laboratory uses advanced instrumentation such as the Applied Biosystems StepOne Plus Real-Time PCR System, which provides precise temperature control and fluorescence detection necessary for reliable qRT-PCR results. The system allows us to monitor amplification efficiency in real-time, ensuring that each reaction follows expected kinetics.
The quantification process involves several steps: RNA extraction from the sample using a combination of mechanical disruption and chemical lysis, followed by reverse transcription into cDNA. The resulting cDNA is then subjected to qRT-PCR analysis using primers specific for norovirus sequences. Amplification products are detected and quantified based on standard curves generated with known concentrations of viral RNA.
The results from our ISO 15216-2 compliant test provide quantitative data that can be used for various purposes, including compliance with regulatory requirements such as the European Union’s Regulation (EC) No 853/2004 on microbiological criteria for food of animal origin. The data also supports decision-making processes in food safety programs and helps mitigate risks associated with norovirus contamination.
Our commitment to quality is reflected not only in our adherence to ISO standards but also in the continuous training of our staff, regular calibration of instruments, and participation in proficiency testing programs organized by recognized bodies. This ensures that our results are reliable and accurate, contributing to the overall integrity of the food safety system.
| Applied Standards |
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| ISO 15216-2: Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) for the detection and quantification of noroviruses in foodstuffs, including bivalve mollusks. |
Applied Standards
The ISO 15216-2 standard is widely recognized for its rigorous guidelines on the use of qRT-PCR technology in foodborne virus detection and quantification. This standard ensures that laboratories conducting these tests adhere to best practices, thereby enhancing the reliability and accuracy of results.
Our laboratory strictly follows ISO 15216-2, which includes detailed protocols for sample preparation, RNA extraction, reverse transcription, qRT-PCR setup, and data analysis. Compliance with this standard not only guarantees high-quality results but also ensures that our findings are compatible with international standards, facilitating seamless communication between laboratories worldwide.
The standard emphasizes the importance of using validated reagents and primers to minimize false positives and negatives, which is crucial for accurate quantification. It also provides recommendations on how to establish and validate internal controls, ensuring consistency across different batches and test runs. By adhering to these guidelines, we can provide clients with confidence in our results.
The ISO 15216-2 standard also highlights the need for proficiency testing programs and regular calibration of instruments. These practices ensure that our equipment remains accurate and up-to-date, contributing to the overall quality of our services. Our commitment to continuous improvement and adherence to international standards underscores our dedication to providing reliable and trustworthy results.
Why Choose This Test
The ISO 15216-2 quantification of norovirus in bivalve mollusks by real-time PCR is an essential service for ensuring the safety and quality of food products. By choosing this test, clients benefit from a comprehensive approach to detecting and quantifying noroviruses, which can lead to enhanced public health protection.
- Compliance with international standards: Adherence to ISO 15216-2 ensures that our results are reliable and consistent with global best practices.
- Precision in quantification: Our advanced instrumentation allows for accurate measurement of viral load, providing quantitative data crucial for regulatory compliance and risk assessment.
- Reliability: By following strict protocols and using validated reagents, we minimize the risk of errors and ensure consistent results across different batches and test runs.
- Rapid turnaround times: Our efficient workflow ensures timely delivery of reports, allowing clients to make informed decisions promptly.
- Expertise: Our team of experts brings extensive experience in food safety testing, ensuring that we provide the highest level of service and support.
The ISO 15216-2 quantification test is particularly beneficial for companies involved in the production and distribution of bivalve mollusks. By incorporating this test into their quality control programs, they can ensure that their products meet rigorous safety standards and contribute to public health.
Moreover, this service supports regulatory compliance with international regulations such as the European Union’s Regulation (EC) No 853/2004 on microbiological criteria for food of animal origin. By providing accurate quantification data, we help clients maintain their reputation and trust in the market.
Customer Impact and Satisfaction
- Enhanced public health protection: By detecting norovirus contamination early, clients can take proactive measures to prevent outbreaks and protect consumers.
- Improved product quality: Accurate quantification ensures that food products meet strict safety standards, enhancing consumer confidence in the brand.
- Increased regulatory compliance: Compliance with international standards such as ISO 15216-2 helps clients avoid legal issues and maintain their market position.
- Competitive advantage: By demonstrating a commitment to food safety and quality, clients can differentiate themselves from competitors in the marketplace.
- Reduced risk of recalls: Early detection and quantification enable timely interventions, minimizing the likelihood of product recalls and associated costs.
We are proud to have earned a high level of customer satisfaction through our dedication to quality and reliability. Our clients trust us to deliver accurate results that contribute to their success in the food industry.
