ISO 23832 GMO Screening in Soybean by Real-Time PCR
Introduction to ISO 23832: ISO 23832:2015 is an international standard that provides a method for the detection and quantification of genetically modified organisms (GMOs) in soybean samples. This service ensures compliance with regulatory requirements and supports the integrity of food supply chains by identifying unauthorized GMO modifications.
Real-Time PCR Technology: Real-time polymerase chain reaction is a widely used technique for detecting specific DNA sequences. In this context, it allows for sensitive and accurate identification of genetically modified soybeans through the amplification of target DNA regions unique to transgenic events.
Sensitivity and Specificity: The method offers high sensitivity, with detection limits as low as 0.1% GMO content in a sample. This precision is critical for ensuring that food products meet regulatory thresholds for GMO presence. Real-time PCR’s specificity ensures accurate identification of the particular GMO event being tested.
Sampling and Preparation: Samples are typically prepared by grinding soybean samples into fine powders, followed by extraction and purification to isolate genomic DNA. This preparatory step is crucial to ensure that the subsequent real-time PCR analysis provides reliable results.
Instrumentation: The test utilizes a real-time PCR machine equipped with appropriate reagents and probes designed for specific GMO events. These instruments are calibrated regularly to maintain accuracy and precision in quantification.
Data Analysis: Data from the real-time PCR instrument is analyzed using software that calculates cycle threshold (Ct) values, which indicate the number of cycles required to reach a predefined fluorescence threshold. The Ct value directly correlates with the amount of target DNA present, allowing for quantitative determination of GMO content.
Regulatory Compliance: This service supports compliance with various national and international regulations that mandate the labeling or prohibition of certain GMO events in food products. By ensuring accurate identification and quantification, ISO 23832 helps companies maintain regulatory integrity and consumer trust.
Scope and Methodology
The scope of this service includes:
- Detection of specific GMO events in soybean samples
- Quantification of the percentage of GMO content present
- Compliance with ISO 23832:2015 standards
- Auditing and validation of testing procedures
The methodology follows a structured approach:
- Sampling and preparation of soybean samples
- Extraction and purification of genomic DNA
- Amplification of target DNA sequences using real-time PCR
- Analysis of data to determine GMO content
This process ensures the accuracy and reliability of results, making it suitable for both quality assurance and regulatory compliance.
Customer Impact and Satisfaction
Our customers benefit from our ISO 23832 GMO Screening in Soybean by Real-Time PCR service in several ways:
- Regulatory Compliance: Ensures adherence to international and national regulations regarding the presence of GMOs in food products.
- Consumer Trust: Maintains consumer confidence through accurate labeling and transparency about product content.
- Risk Management: Identifies potential risks early, allowing for corrective actions before they impact the supply chain or market reputation.
- Quality Assurance: Provides a robust method to verify the purity of soybean products, ensuring high-quality standards are met.
We strive to exceed customer expectations by delivering accurate and timely results. Our team works closely with clients to understand their specific needs and tailor our services accordingly, enhancing overall satisfaction and trust in our testing capabilities.
Competitive Advantage and Market Impact
- Regulatory Leadership: By staying ahead of regulatory changes and maintaining compliance, we provide a competitive edge to our clients.
- Market Differentiation: Our precise GMO detection capabilities allow companies to differentiate their products in the market by ensuring they meet or exceed the strictest standards.
- Innovation Support: We assist R&D teams in identifying and validating new GMO events, supporting innovation in genetic modification technologies.
- Supply Chain Integrity: Ensuring the integrity of the supply chain through accurate testing helps prevent contamination and maintains brand reputation.
These advantages contribute to a stronger market position for our clients by ensuring they are well-prepared to meet future challenges and opportunities in the food industry.
