ISO 21570 Quantitative Real-Time PCR Analysis of GMOs
The ISO 21570 standard provides a robust framework for the quantitative real-time polymerase chain reaction (qPCR) analysis of genetically modified organisms (GMOs). This service is essential for ensuring compliance with international regulatory requirements, especially in the food and feed sectors where GMO content can significantly impact product safety and labeling.
Our laboratory employs state-of-the-art qPCR technology to accurately quantify the presence of specific genetic modifications. The process begins with a thorough sample preparation step that involves extracting DNA from raw materials or processed foods. This extraction is critical as it ensures accurate quantification by minimizing interference from non-target nucleic acids.
Following extraction, the prepared samples undergo qPCR analysis using primers and probes specifically designed for target GMOs. These reagents are selected based on the latest genetic data to ensure high specificity and sensitivity. The qPCR instrument then amplifies the target DNA sequences in a highly controlled environment, allowing us to determine the exact percentage of GMO content in the sample.
The results from our ISO 21570-compliant analysis are presented with detailed reports that include raw data, calculated percentages, and compliance status. These reports are invaluable for quality managers, R&D engineers, and procurement teams who need accurate information to make informed decisions about product development, sourcing, and labeling.
The reliability of our service is further enhanced by strict adherence to ISO standards and continuous calibration of our instruments. This ensures that all results are consistent and repeatable across multiple analyses. Our team of experts uses their deep knowledge of both the technical aspects of qPCR and the regulatory landscape to provide comprehensive support to clients.
One key advantage of using this service is the ability to detect even trace levels of GMOs, which can be crucial for maintaining product integrity in markets with strict labeling laws. Additionally, our service helps food manufacturers comply with global regulations such as those enforced by the European Union and the United States Department of Agriculture (USDA).
The following list highlights some critical aspects of our ISO 21570 qPCR analysis:
- High specificity primers for target GMOs
- Robust sample preparation techniques
- Calibrated qPCR instruments
- Detailed reporting with raw data and compliance assessments
- Compliance with international standards (ISO 21570)
Scope and Methodology
The scope of our ISO 21570 service encompasses the analysis of genetically modified organisms in various food and feed products. This includes crops such as maize, soybean, canola, and sugar beet, which are commonly modified for traits like pest resistance or increased yield.
The methodology involves several key steps: sample collection, DNA extraction, qPCR amplification, and data analysis. Each step is meticulously documented to ensure traceability and accuracy of results.
For sample preparation, we use optimized protocols that minimize potential contamination and ensure consistent results. The extracted DNA undergoes qPCR analysis using a validated method that ensures accurate quantification of the target GMOs. After amplification, the data are analyzed using specialized software to calculate the percentage of GMO content in the sample.
The methodology is based on ISO 21570 and other relevant international standards, which provide clear guidelines for each stage of the process. Our laboratory staff undergoes rigorous training to ensure they adhere strictly to these protocols, ensuring that our results are both reliable and reproducible.
Quality and Reliability Assurance
- Calibration: Our qPCR instruments undergo regular calibration to ensure precision and accuracy in all analyses.
- Validation: All assays are validated using certified reference materials (CRMs) to ensure they meet the required performance criteria.
- Data Validation: We use statistical methods to validate the data, ensuring that any detected GMOs are accurately quantified.
Competitive Advantage and Market Impact
Our ISO 21570 service offers significant competitive advantages in the food and feed sectors. By providing accurate and reliable GMO content analysis, we enable our clients to meet stringent regulatory requirements and maintain product integrity.
In today's global market, consumers are increasingly concerned about the origin of their food products. Our service helps companies demonstrate transparency by accurately labeling their products with GMO content information. This is particularly important for those operating in markets that have strict labeling laws regarding genetically modified organisms.
Our clients also benefit from our expertise in interpreting results and providing strategic advice on how to use this information to improve product quality and compliance. By staying ahead of regulatory changes, companies can ensure they are always compliant with the latest standards.
