ISO 20654 Vitamin B1 (Thiamine) Quantification in Baby Foods
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ISO 20654 Vitamin B1 (Thiamine) Quantification in Baby Foods

ISO 20654 Vitamin B1 (Thiamine) Quantification in Baby Foods

ISO 20654 Vitamin B1 (Thiamine) Quantification in Baby Foods

The quantification of vitamin B1 (thiamine) in baby foods is critical for ensuring product safety and compliance with international standards. ISO 20654 provides a standardized method to accurately measure the thiamine content, which plays a crucial role in infant nutrition by supporting energy metabolism.

Thiamine deficiency can lead to serious health issues such as beriberi and Wernicke-Korsakoff syndrome. Ensuring adequate levels of this vitamin is essential for the healthy development of infants. The test method outlined in ISO 20654 is designed to be precise, accurate, and reproducible, allowing laboratories to reliably quantify thiamine across various baby food products.

The process involves several steps: sample preparation, extraction, derivatization (if necessary), and analysis using high-performance liquid chromatography (HPLC) or similar techniques. The method is validated for use with different types of baby foods, including formula, cereal-based products, and mixed-texture meals.

Compliance with ISO 20654 ensures that manufacturers can demonstrate adherence to dietary recommendations set by health authorities such as the World Health Organization (WHO) and the Food and Agriculture Organization (FAO). This compliance is particularly important for infant formula, where accurate nutrient content is vital for infant development.

The test method also helps in identifying potential adulteration or contamination issues. By quantifying thiamine levels, laboratories can ensure that the products meet the specified nutritional claims on labels and are free from harmful contaminants.

For quality managers and compliance officers, this service is crucial for maintaining product integrity and consumer trust. R&D engineers benefit from accurate data to optimize formulas and improve product consistency. Procurement teams can use these results to verify supplier performance and ensure consistent product quality across batches.

Sample Preparation Treatment Steps Analytical Method
Grind or homogenize samples thoroughly. Add extraction solvent and vortex mix for 10 minutes. Inject into HPLC system with UV detection.
Precisely weigh a known quantity of sample. Perform derivatization if using liquid chromatography. Analyze under optimized conditions to achieve baseline separation.

The use of ISO 20654 ensures that all stakeholders in the food and feed sector can rely on consistent, reliable test results. This standard is widely recognized for its ability to provide accurate quantification, making it a preferred choice for laboratories around the world.

Why It Matters

The importance of accurately quantifying vitamin B1 in baby foods cannot be overstated. Ensuring that babies receive the correct amount of this essential nutrient is vital for their overall health and development. A deficiency can lead to severe health issues, while excess levels may also have adverse effects.

Accurate quantification helps manufacturers meet labeling requirements set by regulatory bodies such as the WHO and FAO. It ensures that products are safe and effective, contributing to public health and consumer confidence. For quality managers and compliance officers, this service provides peace of mind knowing that their products meet international standards.

R&D engineers can use these results to refine formulas and improve product consistency. Procurement teams benefit from verified supplier performance data, ensuring consistent product quality across batches. The reliability of ISO 20654 quantification is essential for maintaining a high standard of infant nutrition.

By adhering to this international standard, laboratories can contribute to the overall health and well-being of infants worldwide, making a significant impact in public health.

Industry Applications

  • In infant formula manufacturing to ensure adequate thiamine levels.
  • In cereal-based products for consistent nutrient content.
  • In mixed-texture meals to verify product integrity.
  • In regulatory compliance checks for imported and exported baby food products.
  • To detect potential adulteration or contamination in baby foods.
Sample Type Recommended Dilution Analytical Range
Infant formula 1:10 dilution with water 0.5-5 mg/L
Cereal-based baby food 1:20 dilution with ethanol 0.2-2 mg/L
Mixed-texture meals 1:30 dilution with phosphate buffer 0.4-4 mg/L

The application of ISO 20654 is widespread across the food and feed sector, particularly in areas focused on infant nutrition. It supports various aspects of product development, quality control, and regulatory compliance.

Use Cases and Application Examples

  1. Manufacturing infant formula to meet international nutritional standards.
  2. Verifying the nutrient content in cereal-based baby foods for consistency.
  3. Detecting potential contamination issues in mixed-texture meals.
  4. Regulatory compliance checks for imported and exported baby food products.
  5. Quality control testing during production processes.
Sample Preparation Method Analytical Instrument Used Expected Outcome
Vortex mixing for 10 minutes with extraction solvent. HPLC equipped with UV detector. Absorbance peak at 325 nm indicating thiamine presence.
Derivatization step if necessary. Reversed-phase column for separation. Detailed quantification report including concentration and recovery rates.

The use of ISO 20654 in these scenarios ensures that baby foods meet the required nutritional standards, contributing to infant health and development. This service is a cornerstone for ensuring product safety and regulatory compliance across the food and feed sector.

Frequently Asked Questions

Is ISO 20654 applicable to all types of baby foods?
Yes, the method is validated for use with infant formula, cereal-based products, and mixed-texture meals. Each type of product has specific preparation steps outlined in the standard.
How long does it take to perform the ISO 20654 test?
The complete process, from sample preparation to final analysis, typically takes around 3 hours. This includes extraction, derivatization (if necessary), and HPLC analysis.
What instruments are required for the ISO 20654 test?
The primary instrument required is an HPLC equipped with a UV detector. Additional equipment includes vortex mixers, micropipettes, and a balance.
How accurate are the results of ISO 20654?
ISO 20654 is designed to provide highly accurate quantification. The method's precision and accuracy have been validated through extensive testing, ensuring reliable results.
Can this service be used for other vitamins besides thiamine?
While ISO 20654 specifically targets thiamine quantification, similar methods can be adapted for other water-soluble vitamins. However, each vitamin requires a specific method tailored to its properties.
Is this service suitable for small laboratories?
Yes, the method is straightforward and can be adapted even in smaller laboratories with basic HPLC equipment. The key is ensuring proper sample preparation and adherence to standard procedures.
How often should this test be performed?
The frequency of testing depends on the production schedule and quality control protocols set by the manufacturer. Regular testing is recommended to ensure consistent product quality.
What happens if a sample does not meet the standard?
Non-compliant samples should be retested and evaluated for potential causes of deviation. Adjustments can then be made to production processes to ensure compliance.

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