EN 16877 Mycotoxin Screening in Juice Products
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EN 16877 Mycotoxin Screening in Juice Products

EN 16877 Mycotoxin Screening in Juice Products

EN 16877 Mycotoxin Screening in Juice Products

The European Standard EN 16877 provides a harmonized method for the screening of mycotoxins in juice products. Mycotoxins are toxic secondary metabolites produced by molds (fungi), which can contaminate various food and feed products, posing significant health risks to consumers.

Food safety is paramount, especially for beverages like juices that have a wide consumer base. Juice products, being highly perishable and prone to microbial contamination, are particularly vulnerable to mold growth leading to mycotoxin accumulation. This standard ensures consistent detection levels of these toxins across different juice types, thereby safeguarding public health.

The procedure outlined in EN 16877 involves extraction, clean-up, and analysis using liquid chromatography with tandem mass spectrometry (LC-MS/MS). It covers a range of mycotoxins including aflatoxin B1, B2, G1, G2; zearalenone; and ochratoxin A, among others.

The testing process begins with proper sample preparation. Juice samples are filtered to remove any particulate matter or sediment that may interfere with the analysis. Extraction solvents like methanol or acetonitrile are used to transfer mycotoxins from the sample matrix into solution form suitable for LC-MS/MS.

After extraction, the sample undergoes cleanup steps such as solid-phase extraction (SPE) cartridges to concentrate and purify the target analytes. This step enhances sensitivity by reducing matrix effects that could otherwise obscure detection signals.

The final step involves high-performance liquid chromatography coupled with mass spectrometry for quantitative analysis of mycotoxins present in the sample. The instrument detects the presence of each toxin based on its unique molecular weight and fragmentation pattern, comparing these against known standards.

  • Methanol
  • Acetonitrile
  • Solid-phase extraction (SPE) cartridges
  • High-performance liquid chromatography (HPLC)
  • Tandem mass spectrometry (MS/MS)

Eurolab Advantages

At Eurolab, we pride ourselves on delivering accurate and reliable mycotoxin screening results. Our state-of-the-art laboratory facilities equipped with advanced LC-MS/MS instrumentation ensure precise detection down to the lowest permissible levels.

We employ highly trained technicians who adhere strictly to EU standards ensuring consistency in all our analyses. Our robust quality management system guarantees adherence to international norms like ISO 17025, thereby providing assurance that every test is conducted under controlled conditions.

Our extensive experience in food and feed testing enables us to provide comprehensive reports tailored specifically for your requirements. Whether you need basic compliance checks or detailed breakdowns of specific compounds, we offer flexible options suited to various business needs.

Environmental and Sustainability Contributions

  • By ensuring food safety through rigorous mycotoxin screening, we help reduce waste associated with contaminated products. This minimizes environmental impact by preventing the disposal of potentially harmful materials.
  • Our services contribute to sustainable agriculture practices by promoting better understanding and management of mold growth in crops used for juice production. This leads to more efficient resource use and reduced chemical treatments.

Use Cases and Application Examples

The application of EN 16877 is broad, encompassing various types of fruit juices such as apple, orange, grape, and cranberry. These beverages are popular worldwide but also carry higher risks due to their direct consumption without further processing.

Quality managers at beverage manufacturers rely on this service for regular testing during production processes. They use the results to implement corrective actions promptly if any contamination is detected early stages of production.

R&D engineers benefit from our services by gaining insights into potential sources and levels of mycotoxin contamination, which helps them develop safer products. Procurement teams can use these findings to select suppliers whose practices align with stringent quality standards.

Frequently Asked Questions

What does EN 16877 cover?
EN 16877 covers the screening of mycotoxins in juice products, including aflatoxin B1, B2, G1, G2; zearalenone; and ochratoxin A.
How long does it take to get results?
Typically, results are available within 5-7 business days from receipt of the sample. However, this can vary based on the complexity of the analysis.
What equipment is used for testing?
We utilize high-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS/MS). The LC-MS/MS system allows us to precisely identify and quantify mycotoxins.
Is there a limit of quantification?
Yes, the limit of quantification varies depending on the specific toxin but generally ranges from 0.1 to 5 μg/kg.
Can you test for other compounds besides mycotoxins?
Certainly! We offer a wide range of additional testing services including pesticide residue analysis, heavy metal screening, and microbiological examinations.
Do you provide certification with your reports?
Yes, all our reports are certified according to ISO 17025 standards. This provides assurance of the accuracy and reliability of our results.
What specimen preparation is required?
For juice samples, we recommend filtering through a 0.45 μm filter prior to extraction. This helps in removing any particulate matter or sediment that might interfere with the analysis.
How often should I schedule testing?
Testing frequency depends on factors like production volume, supply chain practices, and specific regulatory requirements. Regular reviews are recommended to maintain compliance with international standards.

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