ALT and AST Enzyme Profiling in Laboratory Animals
The analysis of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) is crucial for assessing the hepatic function and integrity in laboratory animals. This test profile provides essential insights into potential liver damage or dysfunction, which can be critical in preclinical studies and toxicological assessments.
ALT and AST are key indicators of hepatocellular injury. Elevated levels suggest a disruption in the normal metabolic processes within the liver cells. In clinical and healthcare testing, these enzymes play a vital role in evaluating drug toxicity, diagnosing hepatic disease, and monitoring therapeutic efficacy. For laboratory animals used in research and development (R&D) environments, accurate enzyme profiling is necessary to ensure that test results are valid and reliable.
The process of ALT and AST enzyme profiling involves the collection of blood samples from laboratory animals, followed by meticulous processing using standardized techniques. The collected serum or plasma must be analyzed promptly to avoid any degradation of enzymes, which can lead to inaccurate results. Our state-of-the-art laboratories employ advanced analytical methods such as colorimetric assays to measure enzyme activity accurately.
The instrumentation used in our testing is calibrated and validated according to international standards, ensuring precision and accuracy. The laboratory adheres strictly to ISO 17025 accreditation criteria, which guarantees the quality of results produced by our team of skilled professionals.
Our service includes not only the analysis but also comprehensive reporting tailored to meet your specific requirements. This report provides detailed information on enzyme levels alongside relevant clinical interpretations that are essential for decision-making in R&D and regulatory submissions.
In conclusion, ALT and AST enzyme profiling is an indispensable tool in the evaluation of hepatic health in laboratory animals. By providing accurate and reliable data, we support pharmaceutical companies, biotech firms, and academic institutions in their pursuit of innovative treatments and safer medications.
Applied Standards
| Standard | Description |
|---|---|
| ISO 15195-3:2016 | Methods of sampling, preparation, storage, and handling of clinical specimens for use in clinical laboratory tests. |
| ASTM E1478-18 | Standard test method for the determination of aspartate aminotransferase (AST) activity by kinetic photometric measurement using L-alanine and pyruvate as substrates in serum or plasma. |
| ISO 9232:1994 | Methods for estimation of the activity of alanine aminotransferase (ALT) in serum by kinetic photometric measurement using α-ketoglutarate and L-alanine as substrates. |
| Parameter | Range (U/L) | Description |
|---|---|---|
| ALT | 0 - 45 U/L | Indicates hepatocellular injury. |
| AST | 0 - 39 U/L | Reflects a range of liver conditions including inflammation and necrosis. |
Eurolab Advantages
At Eurolab, we pride ourselves on offering unparalleled expertise in clinical and healthcare testing. Our team comprises highly qualified professionals with extensive experience in the analysis of ALT and AST enzymes. Our laboratories are equipped with cutting-edge technology that ensures precise and reliable results.
We adhere strictly to international standards such as ISO 17025, ensuring our procedures meet the highest quality and reliability benchmarks. This accreditation provides assurance that all aspects of our testing process—from sample preparation to final reporting—are conducted rigorously and consistently.
Our commitment to excellence is further demonstrated through continuous training for staff, regular calibration and maintenance of equipment, and adherence to stringent protocols. These measures guarantee the accuracy and consistency required in laboratory animal testing.
In addition to our technical capabilities, we offer flexible services tailored to meet your specific needs. Whether you require routine testing or specialized analyses, our team is committed to providing prompt, accurate results that support your research objectives.
Why Choose This Test
The significance of ALT and AST enzyme profiling cannot be overstated in the realm of clinical and healthcare testing. These enzymes serve as critical biomarkers for assessing liver health and identifying potential issues before they escalate into more serious conditions.
For pharmaceutical companies, this test is essential in drug development stages to ensure that new compounds do not cause hepatotoxicity. By monitoring ALT and AST levels during clinical trials, researchers can identify any adverse effects early on and take appropriate action.
In academic settings, this profiling aids in understanding the mechanisms behind various liver diseases and how different treatments impact hepatic function. This knowledge is invaluable for advancing medical research and improving patient outcomes.
Moreover, regulatory bodies often require such tests as part of drug approval processes. Our service ensures that you meet these stringent requirements with confidence. By choosing Eurolab, you are investing in a robust testing platform that supports your commitment to quality and integrity.
