OECD 202 Daphnia Acute Immobilisation Test in Groundwater
The OECD (Organisation for Economic Co-operation and Development) 202 Daphnia Acute Immobilisation Test is a critical tool used to assess the acute toxicity of substances, especially those intended for use in or around aquatic environments. This test method evaluates the impact of contaminants on Daphnia magna, a key species in freshwater ecosystems, by measuring their ability to cause immobilization within a short timeframe.
The OECD 202 test is widely recognized and validated globally, ensuring consistent and reliable results across different laboratories. It plays a pivotal role in environmental compliance for industries such as agriculture, water treatment, and chemical manufacturing. The primary goal of this test is to provide quantitative data on the toxicity levels that can help in making informed decisions regarding substance use and potential risks to aquatic life.
The methodology involves exposing adult Daphnia magna to samples containing the substance under investigation. Observations are made over a period of 48 hours, during which the number of individuals exhibiting immobilization is recorded. The test results provide a clear indication of whether or not the substance poses an immediate threat to aquatic ecosystems.
For industries handling groundwater and aquifer resources, this test ensures compliance with stringent environmental regulations. By identifying potential risks early on, companies can implement necessary measures to mitigate these impacts, thus protecting both natural resources and public health. This service is particularly valuable for organizations involved in:
- Water treatment facilities
- Agricultural product development
- Chemical manufacturing processes
- Environmental consulting firms
The OECD 202 test aligns with international standards such as ISO and OECD guidelines, ensuring its reliability and acceptance in regulatory frameworks worldwide. This makes it an essential component of comprehensive environmental management plans.
| Key Parameters | Test Conditions |
|---|---|
| Daphnia magna | Adult specimens used for testing |
| Temperature | 20 ± 1°C |
| pH | 7.0 ± 0.5 |
| Test Duration | 48 hours |
| Sample Volume | 20 mL per test vial |
This detailed approach ensures accurate and reproducible results, which are crucial for regulatory compliance and scientific accuracy. The OECD 202 Daphnia Acute Immobilisation Test is not just a laboratory procedure but an integral part of safeguarding environmental health.
Scope and Methodology
The scope of the OECD 202 Daphnia Acute Immobilisation Test includes assessing the acute toxicity of water samples, particularly those intended for use in or around aquatic environments. This test is applicable to a wide range of substances including pesticides, herbicides, heavy metals, and other chemicals that might find their way into groundwater systems.
The methodology involves preparing the test samples by diluting them with deionized water according to specified ratios. The prepared solutions are then introduced into individual vials containing adult Daphnia magna. These vials are kept under controlled conditions regarding temperature and pH levels, which are critical for the survival of the organisms.
Observations are made every 24 hours over a period of 48 hours to monitor any signs of immobilization or lethality among the test subjects. The number of immobile Daphnia per vial is recorded as an indicator of toxicity levels. This data helps in determining the LC50 (lethal concentration for half) and EC50 (effective concentration for half) values, which are essential parameters for understanding the potential risks associated with the tested substances.
The acceptance criteria for this test include observing a minimum of 10 Daphnia per vial. The results are considered valid if at least three replicate tests yield consistent data within ±20% of each other. This ensures reliability and reproducibility, which are crucial for regulatory compliance and scientific accuracy.
| Sample Preparation | Test Conditions | Observation Frequency |
|---|---|---|
| Dilution of samples with deionized water | 20 ± 1°C, pH 7.0 ± 0.5 | Every 24 hours for 48 hours |
| Vial Volume | 20 mL per vial | |
| Number of Daphnia | 10 per vial | |
| Replicate Tests | Minimum of three replicates |
This comprehensive approach ensures accurate and reliable results, which are essential for regulatory compliance and scientific accuracy. The OECD 202 Daphnia Acute Immobilisation Test is not just a laboratory procedure but an integral part of safeguarding environmental health.
Why Choose This Test
The OECD 202 Daphnia Acute Immobilisation Test offers several advantages that make it an indispensable tool for industries dealing with groundwater and aquifer monitoring. Firstly, its global recognition ensures consistent and reliable results across different laboratories. This standardization is crucial in maintaining credibility and ensuring compliance with international regulations.
Secondly, the test provides quantitative data on the toxicity levels of substances, which can help in making informed decisions regarding substance use and potential risks to aquatic life. By identifying potential risks early on, companies can implement necessary measures to mitigate these impacts, thus protecting both natural resources and public health.
The OECD 202 Daphnia Acute Immobilisation Test aligns with international standards such as ISO and OECD guidelines, ensuring its reliability and acceptance in regulatory frameworks worldwide. This makes it an essential component of comprehensive environmental management plans. It is particularly valuable for organizations involved in water treatment facilities, agricultural product development, chemical manufacturing processes, and environmental consulting firms.
Moreover, the test ensures that companies are meeting their legal obligations by providing data that can be used to support compliance with environmental regulations. By using this test, industries can demonstrate their commitment to sustainability and corporate social responsibility.
Environmental and Sustainability Contributions
The OECD 202 Daphnia Acute Immobilisation Test plays a crucial role in promoting environmental protection and sustainability by providing accurate data on the toxicity levels of substances. This information is vital for industries involved in groundwater and aquifer monitoring, as it helps them make informed decisions regarding substance use and potential risks to aquatic life.
By identifying potential risks early on, companies can implement necessary measures to mitigate these impacts, thus protecting both natural resources and public health. This proactive approach not only ensures compliance with environmental regulations but also promotes a sustainable future by minimizing the adverse effects of human activities on ecosystems.
The OECD 202 Daphnia Acute Immobilisation Test is part of comprehensive environmental management plans that aim to safeguard environmental health. By using this test, industries can demonstrate their commitment to sustainability and corporate social responsibility. This contributes to a healthier planet by promoting the responsible use of resources and reducing pollution.
The results of this test are valuable not only for regulatory compliance but also for research purposes. The data provided can be used to develop new products or processes that have minimal environmental impact, further contributing to sustainable development goals. By incorporating this test into their operations, companies are taking a proactive stance in protecting the environment and promoting sustainability.
