Flow Cytometry Bioanalysis Testing
In the pharmaceutical sector, bioanalytical testing plays a pivotal role in ensuring drug safety and efficacy. Flow cytometry is a powerful analytical technique that allows for the rapid analysis of cells or particles suspended in a fluid as it passes through a laser beam. This service focuses on flow cytometric techniques applied to bioanalysis within the pharmaceutical industry, where precise quantification and characterization are essential.
The flow cytometer measures physical and chemical properties of particles, such as size, volume, granularity, and fluorescence intensity. These measurements can be used to assess cell populations or identify specific cellular markers that are indicative of drug response or toxicity. The technique is particularly useful in bioanalytical testing because it provides detailed insights into the behavior of cells under various conditions.
For pharmaceutical applications, flow cytometry is often used for:
- Determination of cell viability and apoptosis
- Analysis of drug-induced changes in cellular morphology
- Evaluation of immune response to administered drugs
- Quantification of protein levels related to pharmacodynamics or pharmacokinetics
The process typically begins with the preparation of cells from biological samples. This involves harvesting cells using appropriate techniques, followed by staining with fluorescent antibodies or other labeling agents that target specific cell markers or proteins. Once prepared, these samples are introduced into the flow cytometer for analysis.
The equipment used in this service includes advanced flow cytometers such as BD FACSCanto II, Sony SH800C, and Cy安
