AOAC 996.06 Conjugated Linoleic Acid Testing in Edible Oils
The analysis of conjugated linoleic acid (CLA) content in edible oils is critical for ensuring product quality, safety, and compliance with international standards. CLA is a naturally occurring fatty acid that exists in two geometric isomers: cis-9, trans-11 (c9t11), which has been associated with potential health benefits, and trans-10, cis-12 (t10c12), which may have adverse effects on human health. This service focuses specifically on the AOAC 996.06 method for quantifying CLA in edible oils using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
The AOAC 996.06 procedure is widely recognized and validated, making it a reliable tool for laboratories to analyze CLA content with precision and accuracy. This method employs a multi-step extraction process followed by LC-MS/MS detection to ensure the accurate quantification of both cis-9, trans-11 (c9t11) and trans-10, cis-12 (t10c12) isomers in complex matrices like edible oils. This service provides a comprehensive approach that caters to the needs of quality managers, compliance officers, R&D engineers, and procurement professionals who require robust data for decision-making.
The AOAC 996.06 method allows for the accurate quantification of CLA content in various types of edible oils, including olive oil, sunflower oil, corn oil, soybean oil, and others. This service ensures that the results meet stringent quality control standards, thereby enhancing product reliability and customer satisfaction.
Quality assurance is paramount in the food industry, especially when dealing with essential nutrients like CLA. The AOAC 996.06 method provides accurate and reproducible data, which are crucial for maintaining compliance with international standards such as ISO, ASTM, EN, IEC, and FDA guidelines.
The precision of this method is further enhanced by the use of high-performance liquid chromatography (HPLC) coupled with mass spectrometry. This combination ensures that even minute amounts of CLA can be detected and quantified accurately. The method also allows for the simultaneous analysis of other fatty acids present in edible oils, providing a holistic view of the oil composition.
The AOAC 996.06 procedure involves several steps: sample preparation, extraction, cleanup, derivatization, and finally, LC-MS/MS detection. Each step is crucial for ensuring accurate results. The first step, sample preparation, includes homogenizing the sample to ensure uniformity. Extraction uses a solvent system that effectively dissolves CLA from the oil matrix. Cleanup removes any interfering compounds, leaving only CLA for analysis.
The derivatization step converts CLA into a more stable form suitable for mass spectrometric detection. Finally, LC-MS/MS is used to separate and identify the different isomers of CLA present in the sample. The method's specificity ensures that only true CLA is detected, minimizing interference from other fatty acids.
The AOAC 996.06 procedure provides a robust platform for quantifying CLA content in edible oils. This service offers precise and accurate results, ensuring compliance with international standards and quality control requirements.
Applied Standards
| Standard | Description |
|---|---|
| AOAC 996.06 | This standard specifies the procedure for the quantification of conjugated linoleic acid in edible oils using liquid chromatography-tandem mass spectrometry (LC-MS/MS). |
Eurolab Advantages
At Eurolab, we offer a range of advantages that set us apart from our competitors. Our team of highly qualified and experienced professionals ensures that every test is conducted with the utmost precision and accuracy. We have state-of-the-art equipment and facilities that meet the highest industry standards, ensuring reliable results.
- Comprehensive testing services for various food products
- Accurate and precise results using advanced instrumentation
- Strict adherence to international standards and guidelines
- Expertise in sample preparation and analysis techniques
- Timely delivery of reports and certificates
- Competitive pricing without compromising on quality
- Dedicated customer support for all queries and concerns
We pride ourselves on providing a seamless testing experience, from initial consultation to final report. Our commitment to excellence ensures that our clients receive the best possible service.
Why Choose This Test
- The AOAC 996.06 method is widely recognized and validated for its accuracy and precision.
- It provides a comprehensive analysis of CLA content, ensuring compliance with international standards.
- The use of LC-MS/MS ensures the detection of even minute amounts of CLA, providing reliable results.
- Our team of experts can provide detailed reports and recommendations based on the test results.
- We offer competitive pricing without compromising on quality.
- We have strict adherence to international standards and guidelines.
- Comprehensive testing services for various food products
- Accurate and precise results using advanced instrumentation
- Strict adherence to international standards and guidelines
- Expertise in sample preparation and analysis techniques
- Timely delivery of reports and certificates
- Dedicated customer support for all queries and concerns
The AOAC 996.06 method is a reliable tool for quantifying CLA content in edible oils, ensuring product quality and safety. This service provides precise and accurate results, meeting the stringent requirements of international standards.
